نتایج جستجو برای: direct fluorescent antibody
تعداد نتایج: 653062 فیلتر نتایج به سال:
Moody, Max D. (Communicable Disease Center, Atlanta, Ga.) and Wallis L. Jones. Identification of Corynebacterium diphtheriae with fluorescent antibacterial reagents. J. Bacteriol. 86:285-293. 1963.-Conditions are described whereby fluorescent-antibody reagents could be prepared and used to identify Corynebacterium diphtheriae in pure and mixed cultures and in clinical materials. The use of O an...
A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy (FNP-IIFM) was developed for the rapid detection of Mycobacterium tuberculosis. An anti-Mycobacterium tuberculosis antibody was used as primary antibody to recognize Mycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy...
r-phycoerythrin (r-pe), a fluorescent protein from phycobiliprotein family, is isolated from red algae. conjugation of antibodies to r-pe facilitates multiple fluorescent staining methods. in the present study polyclonal antibodies and polyclonal f(ab')2 fragment antibodies were conjugated to r-pe by two different methods. the efficiency of the methods was evaluated using immunocytochemistry (i...
An accelerated, direct immunofluorescent-antibody procedure was developed for the detection of Salmonella in food products. This method includes pre-enrichment and selective enrichment but eliminates many of the washing and smear treatments present in existing methods. Commercially available fluorescein-conjugated somatic antiserum was used in comparing this method with conventional culture, bi...
Over the past decade, there has been an explosion in the use of molecular tests to diagnose and manage infectious diseases. HIV is a prime example of an infectious agent whose diagnosis at least in the acute stage, susceptibility testing, and management are all dependent on molecular diagnostics. The ability to accurately diagnose a plethora of respiratory pathogens quickly, simply, and relativ...
Antisera to ten strains of mycobacteria in the Mycobacterium avium-Mycobacterium intracellulare group were obtained by injecting rabbits with ultraviolet light-killed cells. The antisera were conjugated with fluorescein isothiocyanate and used in the direct fluorescent antibody test. Individual antisera reacted specifically with the mycobacterial serotype used to produce them. The antisera were...
Vibrio cholerae persists in aquatic environments predominantly in a nonculturable state. In this study coccoid, nonculturable V. cholerae O1 in biofilms maintained for 495 days in Mathbaria, Bangladesh, pond water became culturable upon animal passage. Culturability, biofilm formation, and the wbe, ctxA, and rstR2 genes were monitored by culture, direct fluorescent antibody (DFA), and multiplex...
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