This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We an...

The arrays of artificial receptors were synthesized and used in the studies of binding of triphenylmethyl dyes. Rate of binding of Brilliant Black was determined by the measurements of decrease of optical density of dye solution. Considering the fast process of removing excess of dye and uniform coloration of receptors, Brilliant Black was evaluated as the most versatile reporter dye for Indica...

The goal of this work is to predict ductile failure pipe-ring notched AISI 316L specimens, where notches mimic the geometry corrosion defects. Uncoupled damage models are used that end. Johnson-Cook and Lou-Huh criteria calibrated. uncoupled calibrated using experimental results from testing specimens. Calibration was achieved a hybrid experimental-numerical approach. Six notch shapes studied. ...

Abstract If not detected early, the cracks in structural components may ultimately result failure of structure. This issue becomes even more critical when component under investigation is a prosthesis placed human body. study presents crack location identification method based on time domain cantilever beam metallic biomaterials (CBMB). The absolute difference between central approximation root...

Goals: understand why hypervolume-based search is that successful understand basic properties of hypervolume indicator Approach: rigorous running time analyses of a hypervolume-based MOEA for (i) approaching the Pareto front (ii) approximating large Pareto fronts (unary) hypervolume indicator (A) = hypervolume/area of dominated part of search space between front A and reference point Pareto-dom...

the output error of the globoidal cam mechanism can be considered as a relevant indicator ofmechanism performance, because it determines kinematic and dynamical behavior of mechanicaltransmission. based on the differential geometry and the rigid body transformations, themathematical model of surface geometry of the globoidal cam is established. then we present theanalytical expression of the ou...