The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...

       The binding of drugs by plasma proteins is an important phenomenon, because it influences the size of the free fraction of the drugs in plasma. In this study the influence of dipyridamole (DP) on the protein binding of propranolol (PL) and PL on the protein binding of DP were studied alone and in the presence of nicotine (NC).        The equilibrium dialysis was employed for protein bin...

Transcription factors bind specifically to their target elements in the genome, eliciting specific gene expression programs. The nuclear factor-?B (NF-?B) system is a family of proteins comprising inducible transcription activators, which play critical role inflammation and cancer. NF-?B members function as dimers with each monomeric unit binding ?B-DNA. Despite available structures various com...

Abstract The detection of intracellular nucleic acids is a fundamental mechanism host defense against infections. dysregulated acid sensing, however, major cause for number autoimmune diseases. In this study, we report that GTPase-activating protein SH3 domain-binding 1 (G3BP1) critical both DNA- and RNA-induced immune responses. We found in human mouse cells, the deletion G3BP1 led to dampened...

Hydrothermal vents are chemosynthetically driven ecosystems and one of the most extreme environments on Earth. Vent communities exhibit remarkable taxonomic novelty at species supra-species levels, over 80% vent endemic. Here, we used mitochondrial DNA to identify biogeographic distribution Munidopsis lauensis heme-binding regions A1-type COX1 from six (including M. lauensis) investigate whethe...

Blue iguanas ( Cyclura lewisi ) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel Helicobacter species GCBI1 was detected in sick and dead iguanas. In the current study, fecal cloacal swab samples were obtained from 25 Through molecular microbiological techniques, cultured feces characterized, whom we propose name cyclurae . This helicobacter had prevalence of 56% ...