AIM The aim of the study was to evaluate the use of global and gene-specific DNA methylation changes as potential biomarkers for gallbladder cancer (GBC) in a cohort from Chile. MATERIAL & METHODS DNA methylation was analyzed through an ELISA-based technique and quantitative methylation-specific PCR. RESULTS Global DNA Methylation Index (p = 0.02) and promoter methylation of SSBP2 (p = 0.01...

AIM To evaluate the genetic instability of 11 fertile and 25 infertile men. METHODS The methodology of sister chromatid exchanges (SCEs) was applied to cultures of peripheral blood lymphocytes, and the levels of SCEss were analyzed as a quantitative index of genotoxicity, along with the values of the mitotic index (MI) and the proliferation rate index (PRI) as qualitative indices of cytotoxic...

methylated and un-methylated control dna is an important part of dna methylation studies. although human and mouse dna methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. in this paper a simple method of generating methylated and un-methylated control dna is described. whole...

A novel method to encrypt data by applying DNA based computing technology is discussed. Binary data may be encoded in DNA strands by use of an alphabet of short oligonucleotide sequence. The encryption of natural DNA and the encryption of DNA encoding binary data are discussed.A pure XOR based DNA computing scheme is also discussed and the limitations of the proposed scheme are observed. Index ...

One important task in the study of genome sequences and mutations is to determine densities of specific nucleotides and codons. The graphical representation of DNA sequences provide a simple way of viewing, storing, and comparing various sequences. In this paper, we first present for each kind of codon, a numerically representation as a 2D coordinate (x,y) and give a 2D graphical representation...

OBJECTIVE To examine the effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Prospective study. SETTINGS Infertility clinic at a tertiary care academic institution. PATIENT(S) Ninety-two infertile men with normal female partners. Sixteen fertile donors served as the control group. INTERVENTION(S) Standard semen ...

Liver tumors were induced in male rats of the Wistar strain by feeding p-dimethylaminoazobenzene mixed into ground rat food. Surgical biopsies were taken before the animals were fed the azo dye, after 18 weeks of feeding, and when the animals were sacrificed. Tumorous liver was classified as cholangioma, trabecula hepatoma, and apparently normal areas. DNA content (by cytophotometry), mitotic a...

Intrigued by our recent finding that the nuclear refractive index is significantly increased in malignant cells and histologically normal cells in clinical histology specimens derived from cancer patients, we sought to identify potential biological mechanisms underlying the observed phenomena. The cell cycle is an ordered series of events that describes the intervals of cell growth, DNA replica...

[*] Prof. C. Fan, Prof. L. He, Prof. Y. Shi, Z. Zhang, C. Li, Y. Li, L. Qian Y. Fu Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 (PR China) E-mail: [email protected]; [email protected] Prof. C. Fan, Prof. J. Hu, Z. Zhang, Y. Wang Laboratory of Physical Biology, Shanghai Institu...

The present studies were initiated to investigate the changes [3H]deoxythymidine labeling index, primer-dependent DNA polymerase labeling index, and S-G2 transition after treatment of T1699 transplantable mouse mammary tumors with Adriamycin (5 mg/kg) and cyclophosphamide (100 mg/kg). Treatment with these agents resulted in intervals of subnormal tumor cell proliferation as indicated by decreas...