Next-generation sequencing (NGS) technologies have revolutionized modern biological and biomedical research. The engines responsible for this innovation are DNA polymerases; they catalyze the biochemical reaction for deriving template sequence information. In fact, DNA polymerase has been a cornerstone of DNA sequencing from the very beginning. Escherichia coli DNA polymerase I proteolytic (Kle...

Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record of the current to yield the DNA sequence. We demonstrate ...

Microbiology has completely transformed in the last 25 years and realization that most microorganisms cannot be grown a pure culture resulted new methods of analyzing genomic diversity. One such method, Metagenomics, refers to study on basis their diversity, acquired directly from environmental samples. It facilitates physiology ecology through vast array sequencing functional techniques. Over ...

The discovery of epigenetic bases has revolutionised the understanding disease and development. Among most studied marks are cytosines covalently modified at 5 position. In order to gain insight into their biological significance, ability determine spatiotemporal distribution within genome is essential. Techniques for sequencing on “next-generation” platforms often involve harsh chemical treatm...