The objectives of the study are to evaluate DNase I serum levels and their correlation with soluble Fas (sFas) and soluble Fas ligand (sFasL) and with cell surface Fas expression in patients with systemic lupus erythematosus (SLE), thus contributing to the dysregulated apoptosis typical of the disease. The methods include the following: Serum DNase I levels in patients and in controls were dete...

Generation of DNA fragments is a hallmark of cell apoptosis and is executed within the dying cells (autonomous) or in the engulfing cells (non-autonomous). The TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labelling) method is used as an in situ assay of apoptosis by labelling DNA fragments generated by caspase-associated DNase (CAD), but not those by the downstream DNase II. In th...

Motivation: Understanding global gene regulation depends critically on accurate annotation of regulatory elements that are functional in a given cell type. CENTIPEDE, a powerful, probabilistic framework for identifying transcription factor binding sites from tissue-specific DNase I cleavage patterns and genomic sequence content, leverages the hypersensitivity of factor-bound chromatin and the i...

Abstract The current study was carried out in order to know the effect of plant metabolism, fertilizer type and michanical stress (soil compaction) factors on content root exudates mungbean Vigna radiate L. maize Zea mays from primary secondary metabolites. Root were collected their contents metabolites such as total sugars, proteins, phenols, flavonoids terpenoids, Hormons (gibberellic, abscis...

DNase I binds in the minor groove of DNA and is used as an enzymatic tool to investigate the interaction of proteins with DNA. Here we show that the major groove located 5-methyldeoxycytidine can enhance or inhibit the cleavage rates of DNA by DNase I. This effect may be caused in part by changes in DNA structure affecting the accessibility of the minor groove of DNA to DNase I.

We describe DNase-capture, an assay that increases the analytical resolution of DNase-seq by focusing its sequencing phase on selected genomic regions. We introduce a new method to compensate for capture bias called BaseNormal that allows for accurate recovery of transcription factor protection profiles from DNase-capture data. We show that these normalized data allow for nuanced detection of t...

Introduction and Aim: Proteus mirabilis is an opportunistic pathogen, infecting humans, through the release of endotoxins enzymes such as urease, hemolysin, protease, DNase etc. One factors contributing to its virulence unusual ability form crystalline biofilms. This study aimed investigate effect Mentha spicata volatile oil on P. biofilm formation. Materials Methods: was isolated from infected...

in this study, extracts and essential oils of black and red pepper and thyme were tested for antibacterial activity against escherichia coli o157:h7 andstaphylococcus aureus. black and red pepper and thyme were provided from iranian agricultural researches center. 2 g of each plant powder was added to 10 cc ethanol 96°. after 24 h, the crude extract was separated as an alcoholic extract and con...