the effects of sodium n-dodecyl sulphate (sds) on the structure of histone hi has been studied by a combination of e:quilibrium dialysis, u.v. spectroscopy ; polyacrylamide gel electrophoresis, protein titration and viscometery techniques using, 2.5 mm phosphate buffer, ph 6.4. the interaction of h, and sds in contrast tomanyothel-protein-sds interactions is organized between v 40 to 70. above ...

a study was made on the interaction between histon h1 and sodium n-dodecyl sulphate (sds) in the presence of sodium arsenate inside a phosphate buffer of ph 6.4, using spectroscopy and equilibrium dialysis at 27 °c. the binding data has been used to obtain the gibbs free energy in terms of a theoretical model based on the wyman binding potential. the binding data hs been analysed in terms of hi...

this study was carried out to determine the effects of gamma irradiation on dry matter (dm) and on crude protein (cp) degradation of rapeseed meal, using nylon bags and sds-page techniques. ruminal nylon bags of either untreated or irradiated rapeseed meal at doses of 25, 50 and 75 kgy were suspended in the rumen of four non-lactating holstein cows for up to 48 h. intestinal crude protein diges...

Capillary electrophoresis (CE) has emerged as a powerful technique for comprehensive physicochemical characterization of monoclonal antibodies (mAbs) well other therapeutic modalities. The method provides high resolution separation and sensitivity analysis biomolecules. CE based techniques such sodium dodecyl sulphate capillary gel (SDS-CGE, also referred to SDS), zone (CZE) imaged isoelectric ...

The time-dependent response of a surfactant hexagonal phase of a sodium dodecyl sulphate/pentanol/cyclohexane/brine system to stepped strain is investigated. The dynamics of the system is found to be governed by strain- and noise-induced yielding of the domains of the system. The effects of the applied strain magnitude and the ionic strength of the brine on the character of the transitions expe...

Hydropericardium syndrome (HPS) agent was purified by sucrose gradient centrifugation. The polypeptides of HPS agent were separated through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and identified by known protein markers. Eight polypeptides having molecular weights ranging from 119.0 to 15.7 KDa were observed. The nucleic acid of HPS agent was extracted and determin...

The influence of spermine on the potassium loss from yeast cells as a consequence of membrane-disturbing actions was investigated. Spermine interfered strongly in the action membrane-active bactericides on yeast cells; this interference resembles the action of certain metal ions. Spermine provides a protection against the positively charged parasosaniline and accomplishes a strong potentiation ...

Thin-layer chromatography of nineteen amines has been performed on silica gel layers with a water-in-oil microemulsion (sodium dodecyl sulphate + water + heptane + n-pentanol, 8.0 g + 8.0 mL + 160.0 mL + 25.0 mL) as mobile phase. The effect of the basicity of the amines on their RF values was examined. The proposed thin layer chromatographic method was used to separate isomers of nitroaniline f...