Cationic reagents are commonly used to facilitate DNA delivery, and transfection experiments are typically initiated in cell culture where the optimal charge ratio is determined. While transfection rates are often enhanced at higher +/- charge ratios, the cellular toxicity associated with the greater amounts of cationic components at elevated charge ratios is often not considered. In addition, ...

Polyvinylidene fluoride/polyvinylpyrrolidone (PVDF/PVP) hollow fiber membranes were fabricated by dry-jet wet-spinning process. The correlated effects of the air gap length and PVP concentration ((1) the air gap length effect at the low and high PVP concentration in the dope solutions, as well as (2) the effect of the PVP concentration at the same air gap length) on the cross-section structure ...

To enable preventive measures to be designed, it is important to identify modifiable distal and proximal factors underlying doping behaviour. This study investigated aspects of the prototype willingness model in relation to doping. A cross-sectional study was conducted involving 729 competitive athletes. Following ethical approval, athletes (mean age= 28.8 ± 10.1 years; 63% male) completed an o...

In many applications, an ability of liposomes to retain drug and then rapidly release it at some later time would be of benefit. In this work, we investigate the ability of cationic large unilamellar vesicles (LUV) to promote rapid release of doxorubicin from anionic LUV. It is shown that the addition of cationic liposomes containing cholesterol, dioleoylphosphatidylethanolamine (DOPE), distear...

We developed a series of statistical potentials to recognize the native protein from decoys, particularly when using only a reduced representation in which each side chain is treated as a single C(beta) atom. Beginning with a highly successful all-atom statistical potential, the Discrete Optimized Protein Energy function (DOPE), we considered the implications of including additional information...

A one-step, mild procedure based on coaxial electrospinning was developed for incorporation and controlled release of two model proteins, BSA and lysozyme, from biodegradable core-shell nanofibers with PCL as shell and protein-containing PEG as core. The thickness of the core and shell could be adjusted by the feed rate of the inner dope, which in turn affected the release profiles of the incor...

the current methods for treatment of cancers are inadequate and more specific methods such as gene therapy are in progress. among different vehicles, cationic liposomes are frequently used for delivery of genetic material. this investigation aims to prepare and optimize dotap cationic liposomes containing an antisense oligonuclotide (asodn) against protein kinase c alpha in non-small cells lung...