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Polytene chromosomes from the salivary gland cells of Drosophila third instar larvae have been used extensively for cytological and genetic studies. Polytene chromosomes have also been used to study transcription because an actively transcribing gene is often manifested as a localized region of chromatin decondensation or “puff”. Several methods have been developed to detect sites of active tra...
background: cationic immune stimulating complexes (pluscoms) are particulate antigen delivery systems. pluscoms consist of cationic immunostimulatory complexes (iscoms) derivatives and are able to elicit in vivo t cell responses against an antigen. objective: to evaluate the effects of pluscoms containing leishmaniamajor antigens (sla) on the type of immune response generated in the murine mode...
Background: Cationic immune stimulating complexes (PLUSCOMs) are particulate antigen delivery systems. PLUSCOMs consist of cationic immunostimulatory complexes (ISCOMs) derivatives and are able to elicit in vivo T cell responses against an antigen. Objective: To evaluate the effects of PLUSCOMs containing Leishmaniamajor antigens (SLA) on the type of immune response generated in the murine mod...
Abstract INTRODUCTION Our group has developed a patented vaccine platform that combines an mRNA-nanoparticle with CXCL9 loaded polyethylene glycol (PEG) hydrogel. The HCM is given SQ and recruits diverse immune cell population to deliver large payload of mRNA. objective these studies was evaluate the effects vaccination on TME. METHODS C57BL/6 mice underwent intracranial implantation KR158 or G...
Abstract RNA vaccines have shown great promise as activators of immune responses against viral pathogens but their efficacy in cancer is unclear. Here, we describe a versatile, personalized mRNA-nanoparticle (RNA-NP) platform that can be customized to produce powerful anti-tumor activation, reprogram the brain tumor microenvironment, and predict vaccine just two days after treatment. Lipid mixt...
Background: DNA vaccines are third generation vaccines which have made promises to combat infectious diseases. Cationic liposomes are used as effective delivery systems for DNA vaccines to generate stronger immunity. Objective: Encapsulation of pcDNA3.1+PA plasmid, encoding protective antigen (PA) of Bacillus anthracis (B. anthracis) into cationic liposomes, and evaluation of its effect on spec...
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