Segregation distorters are curious, evolutionarily selfish genetic elements, which distort Mendelian segregation in their favor at the expense of others. Those agents include gametocidal factors (Gc), which ensure their preferential transmission by triggering damages in cells lacking them via chromosome break induction. Hence, we hypothesized that the gametocidal system can be adapted for chrom...

Doubled haploid (DH) technology, which is used for rapidly purifying genetic resources, is a key technology in modern maize breeding. The present study evaluated the tissue culture characteristics of maize haploid coleoptile sections, in order to provide a new way of haploid doubling. With 20 combinations of haploid coleoptile sections, obtained by hybridization within Reid, Tangsipingtou, and ...

As large-scale DNA sequencing technologies become more efficient and cheaper, the genomic DNAs of more and more plants are being sequenced, assembled, and annotated. These complete sequences are extremely valuable for the identification of specific genes associated with important phenotypes. The purpose of this document is to propose an efficient strategy for an international effort to sequence...

Haploids and doubled haploids are invaluable for basic genetic studies and in crop improvement. A novel method of haploid induction through genetic engineering of the Centromere Histone Protein gene, CENH3, has been demonstrated in Arabidopsis. The present study was undertaken to develop haploid inducer (HI) lines of Brassica juncea based on the principles elaborated in Arabidopsis. B. juncea w...

The needs of a growing human population require rapid and efficient development of improved cultivars by plant breeders. The doubled haploid (DH) technology enables generating completely homozygous lines in a single step and, thus, is central to modern genetics and breeding approaches. Rapid and reliable identification of seeds with a haploid embryo after in vivo haploid induction is elementary...

HE spontaneous origin in 1926 of a tomato haploid in Lycopersicon Tescu len lun Mill. (LINDSTROM 1929) provided the source material of these investigations. This haploid when doubled asexually by the decapitation-callus method becomes an absolutely homozygous diploid ; and doubling this in turn results in the homozygous tetraploid. Selection experiments have been in progress since 1926 in the h...