RNA viruses encode an RNA-dependent RNA polymerase (RdRp) that catalyzes the synthesis of their RNA(s). In the case of positive-stranded RNA viruses belonging to the order Nidovirales, the RdRp resides in a replicase subunit that is unusually large. Bioinformatics analysis of this non-structural protein has now revealed a nidoviral signature domain (genetic marker) that is N-terminally adjacent...

A total of 51 sera from a migratory population of Burchell's zebras (Equus burchelli) were collected in the Serengeti National Park (Tanzania) between 1999 and 2001 to assess levels of exposure to equine herpesvirus types 1, 2, 4, 9 (EHV-1, -2, -4, -9), EHV-1 zebra isolate T965, and equine arteritis virus (EAV). Using virus-specific neutralizing antibody tests, seroprevalence was high for EHV-9...

Phosphorus (P) biogeochemical processes in wetlands are influenced by the chemical composition of both recently accreted and native soils. The evaluation influencing P transformations requires an understanding participating components. Here, we use X-ray diffraction (XRD), scanning electron microscopy (SEM)/energy-dispersive fluorescence spectroscopy (EDS), absorption near-edge (P-XANES), micro...

Historic phosphorus (P) loading from agricultural areas has been identified as one of the major causes for ecological changes occurring in the Florida Everglades. The restoration plan for the Everglades includes construction of large stormwater treatment areas (STAs) to intercept and treat this relatively high nutrient water down to very low total P (TP) concentrations. One such STA has been in...

MOTIVATION Molecular biology databases have been proliferating rapidly. Their heterogeneity and complexity pose a great challenge to efforts in database interoperation. To minimize the efforts of interoperating heterogeneous databases, it is useful to develop a system that lets a user of a particular genomic database access another related database as if the latter is structurally similar to th...

A semen sample from a stallion infected during the 2010 equine arteritis virus (EAV) outbreak was received for viral isolation prior to castration of the animal. The virus was identified using a polyclonal antibody immunofluorescence test. Reverse-transcription polymerase chain reaction (RT-PCR) was used to amplify a region of the GP5 gene with primers GL105F and GL673R. The PCR products were p...

The pathogenesis and immune response of pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) are not completely understood. PRRSV, along with equine viral arteritis (EAV), lactate dehydrogenase elevating virus of mice (LDV), and simian hemorrhagic fever virus (SHFV), are members of the genus Arteriviridae. This review summarizes the similarities and the differences fou...

Equine viral arteritis (EVA) is a contagious viral disease of equids caused by equine arteritis virus (EAV), an RNA virus that is classified in the family Arteriviridae. The virus is found in horse populations in many countries worldwide. EVA cannot be differentiated clinically from a number of other respiratory and systemic equine diseases. While laboratory-confirmed outbreaks of EVA have been...