Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Dir...

objective(s): there are several scolicidal agents for inactivation of hydatid cyst protoscolices during surgery, but most of them are associated with adverse side effects such as sclerosing cholangitis and liver necrosis. the present study was aimed to evaluate scolicidal effects of various extracts of nigella sativa seeds againstprotoscoleces of hydatid cyst in an in vitro model. materials and...

despite the establishment of extensive and successful control programmes in some countries or regions, echinococcus granulosus still has a very wide geographical distribution. mature e. granulosus (n = 120) obtained from parasitology department, school of veterinary medicine, ferdowsi university of mashhad (iran). soluble protein of whole body of parasite was prepared by freeze-thawing in liqui...

background: cystic echinococcosis (ce), a zoonotic parasitic infection caused by the metacestode (larvae) stage of dog tapeworm echinococcus granulosus and recognized as a major economic and public health concern in the world. this study aimed to investigate the in vitro scolicidal effect of methanolic extract of berberis vulgaris l. roots and its main compound, berberine against protoscoleces ...

background: antigen b (agb) is frequently used for immuno-diagnosis of hu-man cystic echinococcosis (ce). echinococcus granulosus agbs show a high degree of genetic variability in different hosts or in different ce endemic areas. the present study aimed to evaluate the genetic polymorphisms of encoding antigen b2 gene (agb2) among different iranian isolates of e. granulosus. methods: a total of...

b ackground: the aim of the present study was to determine the molecular characteristics of echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using pcr- rflp of its1- rdna analysis in golestan province, northern iran. m ethods: e . granulosus isolates from human patients infected w...

background: the present study was aimed to investigate molecular diversity of echino-coccus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in iran . methods: forty seven human hydatid cysts were collected through surgery from two hospitals in tehran during 2010-2012. to determine the fertility of protoscoleces, the cyst fluids were subjecte...

background: hydatidosis or cystic hydatid disease is one of the most important diseases in human and animals. identification of strains is important for improvement of control and prevention of dis-ease. the aim of this study was to determine the strains isolated from human and domestic animals in ilam province, iran, using pcr-rflp method. methods: respectively, 30 and 4 animal and human hydat...

BACKGROUND Human cystic echinococcosis (CE) is highly endemic in the Tibetan regions of Sichuan where most families keep guard dogs and where there are considerable numbers of ownerless/stray dogs. Strong Buddhist beliefs do not allow for elimination of stray dogs, and many strays are actually fed and adopted by households or monasteries. On account of the high altitude (3900-5000 m), pasturage...

results in this case, the protoscolicidal effect of artemether and its combinations was significantly (p < 0.05) higher than other groups; the maximum protoscolicidal effect was found with 200 μg/ml of artemether after 4 days whereas albendazole killed protoscoleces on the 7th day post-incubation. surprisingly, the incubation of protoscoleces with artemisinine exhibited promising results, as on...