The naturally occurring polyamines, putrescine, spermidine and spermine, have been implicated in many growth processes and in differentiation (Cohen, 1971; Bachrach, 1973; Russell & Durie, 1978). Their concentrations and the activity of their biosynthetic enzymes increase significantly in proliferating cells (Tabor & Tabor, 1976; Janne & Raina, 1968) and neoplastic tissues (Bachrach, 1978; Russ...

This study was designed to investigate the effects of polyamines on mechanical contraction and voltage-dependent calcium current (VDCC) of guinea-pig gastric smooth muscle. Mechanical contraction and calcium channel current I(Ba) were recorded by isometric tension recording and whole-cell patch clamp technique. Spermine, spermidine and putrescine inhibited spontaneous contraction of the gastric...

It is evident that di and polyamines are intimately related to growth processes (1-3). Actively dividing mammalian cells contain higher levels of polyamines than slowly growing or quiscent cells (4,5). Polyamine levels rise In different malignancies ((3,7). On the other hand, activity of Diamine oxidase (DAO; EC 1.4.3.6) increases in human carcinomatous growths as well as in experimental neopla...

The relationship between gross domestic product (GDP) and dietary profile, with a focus on polyamine intake, was investigated in 35 Asian countries. Data on food supply, GDP, and health condition were collected from databases of the United Nations, the International Monetary Fund, and the World Health Organization, respectively. The amount of polyamine intake from food was estimated using polya...

The polyamine path of Neurospora crassa originates with the decarboxylation of ornithine to form putrescine (1,4-diaminobutane). Putrescine acquires one or two aminopropyl groups to form spermidine or spermine, respectively. We isolated an ornithine decarboxylase-deficient mutant and showed the mutation to be allelic with two previously isolated polyamine-requiring mutants. We here name the loc...

A transport system for polyamines was studied with both intact cells and membrane vesicles of an Escherichia coli polyamine-deficient mutant. Polyamine uptake by intact cells and membrane vesicles was inhibited by various protonophores, and polyamines accumulated in membrane vesicles when D-lactate was added as an energy source or when a membrane potential was imposed artificially by the additi...

Polyamines (putrescine, spermidine, and spermine) are present in all higher eukaryotic cells and are essential for cell growth, differentiation and apoptosis. Sharing common precursor with polyamines, nitric oxide (NO) is associated with myocardial ischemia/reperfusion injury by the generation of peroxynitrite. Although polyamines have been implicated in tissue ischemia injury, their metabolism...

Nitric oxide (NO), an endogenous signaling molecule, is involved in various physiological processes and stress responses in plants. In the present research, Pyrodwarf pear (Pyrus communis) rootstocks were grown by nutrient solution to investigate the effects of sodium nitroprusside (SNP) application as an NO donor at 0, 0.1, 0.5, and 1 mM levels on plant stress tolerance, content of ma...

An ion-exchange chromatographic procedure has been developed for the separation and fluorometric detection of the urinary polyamines 1,3-diaminopropane, putrescine, spermidine, cadaverine, and spermine. The polyamines were separated on a 15 x0.45 cm cation-exchange column (70”) employing a combined pH-salt gradient. The separation conditions were designed to enable utilization of the sensitive ...

This paper demonstrates for the first time that plant metabolites of the phenylamide type, conjugates of putrescine with hydroxycinnamic acids (p-coumaric, caffeic and ferulic), possess 1O2 quenching properties. Data were obtained confirming that their acidic parent compounds were also able to quench 1O2, as did polyamines (putrescine, spermidine and spermine), and that this ability depends on ...