Spontaneously transformed mouse cell lines heterozygous for electrophoretic markers have been studied to determine the relationship between gene dosage and phenotype. It is shown that a clone with an electrophoretic pattern for glucosephosphate isomerase of three bands in a ratio of 4A:4AB:1B contains three copies of chromosome 7, which carries the gene for this enzyme. A clone from a different...

We present a procedure for forming a poly(ethylene glycol) (PEG) trimethoxysilane self-assembled monolayer (SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single assembly step and prevents biofouling on silicon and gold surfaces. The SAM is used to coat microelectrodes patterned with standard, positive-tone lithography. Using the microtubule as an example, we ...

Instigated by an increase in serious human Candida infections and aided by advances in technology, there has been renewed interest in the study of the epidemiology of fungal infections. Among the newer techniques available, contour-clamped homogeneous electric field (CHEF) electrophoresis has shown great promise as a tool for typing strains of Candida albicans. However, few studies have address...

A selected group of 308 sera were analyzed by capillary electrophoresis (CE), agarose gel electrophoresis (AGE), and cellulose acetate electrophoresis (CAE) and evaluated for abnormalities that would suggest the presence of a monoclonal protein. The sensitivity (an electrophoretic abnormality in sera that contained a monoclonal protein) and specificity (a normal electrophoretic pattern in sera ...

The study was carried out to identify muscle protein patterns and gut protease activity of rohu (Labeo rohita), catla (Catla catla), climbing perch (Anabas testudineus) spotted snakehead (Channa punctatus). Twelve percent Polyacryleamide substrate SDS-PAGE done visualize the enzyme after casein hydrolysis pattern species. Three alkaline proteases different molecular weight were observed in rohu...

Effect of various NaCl treatments (0, 50, 100, 200, and 300 mM) at different growth and development stages (tillering, boot swellen, flowering and anthesis) in two wheat cultivars (Ghods: salt – sensitive, Boolani: salt – resistant) on SDS-PAGE electrophoretic pattern of leaf proteins was studied under greenhouse conditions. Generally, in response to salinity treatments, the decrease in protein...

porins from salmonella typhi 0-901 and salmonella typhimurium ra-30 were characterized and compared. the elution profile of porins from these salmonella species on sepharose-48 and hplc appear to be very similar. the findings were confirmed by the electrophoretic pattern which showed three types of porins, i.e. ompc, ompd and ompf in both species. these porins appear to be similar, if not ident...

Glutamate dehydrogenase of several different plants was resolved by polyacrylamide gel electrophoresis into separate molecular forms and the isoenzymic patterns detected by the tetrazolium technique were compared. The number of isoenzymes and their electrophoretic mobilities varied among the different plants studied. The isoenzymes were found to have the same coenzyme specificity and to localiz...

The electrophoretic mobility distributions of hairy cells, normal monocytes. CLL, and normal lymphocytes isolated from blood were determined by electrophoretic light scattering. Values obtained for hairy cells, 1.52 X 10(-4) cm2/V . sec, were indistinguishable from that of normal monocytes. The mobility of CLL lymphocytes was similar to that of normal B cells. After exposure to neuraminidase, h...

Porins from Salmonella typhi 0-901 and Salmonella typhimurium Ra-30 were characterized and compared. The elution profile of porins from these salmonella species on Sepharose-48 and HPLC appear to be very similar. The findings were confirmed by the electrophoretic pattern which showed three types of porins, i.e. OmpC, OmpD and OmpF in both species. These porins appear to be similar, if not ...