A method of fractionation of proteins in solution in which an electrophoretic adaptation of the principles of the Clusius column is utilized was suggested by Kirkwood in 1941 (1) and tested experimentally by Nielsen and Kirkwood (2) several years later. Recently an electrophoresis-convection apparatus of improved design has been described and successfully used in the fractionation of the pseudo...

A patient with hypophosphatasia and her relatives, including an affected brother, were studied with regard to serum alkaline phosphatase levels, phosphoethanolamine excretion, leucocyte alkaline phosphatase, blood group genes, and alkaline phosphatase distribution in the serum protein fractions. The pattern of alkaline phosphatase distribution in the serum protein fractions was normal both in t...

There have been several indications (1, 2) that the amylase found in serum is derived from sources other than the pancreas or salivary glands. For this reason we wished to determine whether any indication of fractionation of serum amylase could be obtained by paper electrophoresis. Although several accounts of the fractionation of amylase in serum are found in the literature, some involving pre...

CASE DESCRIPTION A 74-year-old woman with toxic nodular goiter, diabetes mellitus, depression, gastroesophageal reflux disease, and hypercholesterolemia had routine serum protein electrophoresis done for the first time. Her medications included metformin, levothyroxine, sertraline, rosuvastatin, and rabeprazole. The patient’s renal function was normal. Serum protein electrophoresis was carried ...

The evaluation of serum proteins and their electrophoretic pattern is a well-established laboratory method in the diagnosis of many diseases in humans, which has replaced the biochemical determination of the concentrations of albumin and the ratio of albumin to globulins. The measurement of serum proteins may be an important diagnostic tool for the detection, diagnosis, and monitoring of variou...

In the preceding paper on the behavior of pooled normal human serum albumin in various buffers at pH 4.0 (1)) it was demonstrated that the electrophoretic pattern of albumin shows a single moving boundary in the presence of the lower fatty acids, excepting formic and acetic acid, and of certain diand tribasic organic acids. This phenomenon is probably a consequence of the interaction between th...

Previous experiments (l-3) have shown that following hypophysectomy the serum globulin concentration of dogs and rats increases and at the same time the serum albumin level falls. In the above studies serum albumins and globulins were separated by salt fractionation (4). With the aim of checking our earlier results and of obtaining more detailed information regarding the changes in serum protei...

The H8 protein is a surface-exposed antigen that is found, among members of the Neisseria genus, primarily on pathogenic species. In this study, the surface exposure of H8 was reassessed by four techniques. Results of slide agglutination, indirect fluorescent antibody binding, absorption of sera with whole gonococci, and immune electron microscopy all confirmed the presence of H8 in the outer m...

It is a well established fact that the circulating proteins of the human are profoundly affected by surgical, accidental and combat trauma. The medical literature on this topic emphasizes the remarkable quantitative and qualitative changes in intracellular and extracellular protein constituents occurring after and in response to injury. Fundamental information concerning these metabolic alterat...