A new technique, the enzyme-linked immunosorbent assay (ELISA) a@ears to have advantages in some areas over the standard indirect hemagglutination (IHA) test for detecting antibody to Qecific F-l antigen of the plague bacillus, Yersinia pestis. In this presentation the authors compare the results of ELISA and IHA testing and discuss possible ways the former could contribute to solving certain p...

A new method for milk sample collection and storage, based on a dried milk sampling technique, is proposed. The method includes application of a whole milk sample to a porous membrane followed by drying. One hundred whole milk samples (dried and liquid) taken on day 21 post insemination were analysed for progesterone by ELISA and results for both dried and liquid samples were well correlated (r...

The heterogeneous etiology of asthma makes its diagnosis complicated. Measurement cytokine levels could be relevant in determining the phenotype, predicting severity, and identifying treatment type. Enzyme-linked immunosorbent assay (ELISA) is one most reliable methods, with high sensitivity specificity. This study aimed to determine accuracy utility interleukin (IL)-13 IL-17 A diagnosing child...

The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future...

Autoimmune bullous diseases (ABD) are a rarely seen group of diseases, of which pemphigus and bullous pemphigoid (BP) are the major groups. Diagnosis is generally based on the combination of clinical features, histopathologic and immunofluorescence (IF) findings, and/or enzyme-linked immunosorbent assay (ELISA). Aims of the work were to determine the value of the innovative BIOCHIP mosaic-based...

background: the current study was conducted to compare elisa with thin layer chromatography (tlc) methods for diagnosis of morphine in the urine. methods: positive urine samples for morphine confirmed by immunochromatographic strips were collected from the imam reza toxicology laboratory, mashhad university of medical sciences, mashhad, iran in 2012 for the current study. then, the collated uri...

The enzyme-linked immunosorbent assay (ELISA) technique is based on the specific recognition ability of the molecular structure of an antigen (epitope) by an antibody and is likely the most important diagnostic technique used today in bioscience. With this methodology, it is possible to diagnose illness, allergies, alimentary fraud, and even to detect small molecules such as toxins, pesticides,...