Pig oocytes and embryos differ from those of other species in having a large quantity of endogenous lipid, a potential role for which has yet to be identified. In the present study, the hypothesis that endogenous triglyceride acts as a metabolic substrate during in vitro maturation and early embryo development was tested. Embryos were produced by in vitro fertilization (IVF) of in vitro-matured...

Abscisic acid (ABA) plays an important role during somatic embryo development and maturation in coniferous species. The purpose of this research was to study ABA utilization by genotypes with different embryo maturation capabilities in interior spruce. Cell lines ISP11 and ISP48 were of high embryo maturation capability. By contrast, the tissue of line ISP16 contained numerous immature embryos,...

INTRODUCTION Embryo defective mutants in Phaseolus vulgaris obtained by Ethyl methanesulfonate (EMS) induction were studied to determine the structural and molecular basis of seed abortion in this species. In the wild-type, the classical pattern of seed development showed coordinated differentiation of the embryo proper, suspensor, endosperm tissue, and seed coat. On the contrarily, EMS mutant ...

BACKGROUND Linoleic acid (LA) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence. OBJECTIVE In the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovine. MATERIALS AND METHODS The expe...

BACKGROUND Since the identity of the embryo is of outmost importance during commercial in vitro embryo production, bovine oocytes and embryos have to be cultured strictly per donor. Due to the rather low yield of oocytes collected after ovum pick-up (OPU) per individual cow, oocyte maturation and embryo culture take place in small groups, which is often associated with inferior embryo developme...

In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility treatment and preservation. However, standard in vitro culture conditions usually increase reactive oxygen species (ROS), which have been implicated as one of the major causes for reduced embryonic developmental competence. It is well-known that higher than physiological levels of ROS triggers granulosa ...

Coagulansin-A (withanolide) is the steroidal lactone obtained from Withania coagulans which belong to Solanaceae family. The present study investigated the effects of coagulansin-A on bovine oocyte maturation and embryo development in vitro. All these oocytes were aspirated from the ovaries obtained from Korean Hanwoo cows at a local abattoir. To determine whether coagulansin-A has beneficial e...

BACKGROUND Adiponectin is an adipokine, mainly produced by adipose tissue. It regulates several reproductive processes. The protein expression of the adiponectin system (adiponectin, its receptors, AdipoR1 and AdipoR2 and the APPL1 adaptor) in bovine ovary and its role on ovarian cells and embryo, remain however to be determined. METHODS Here, we identified the adiponectin system in bovine ov...

although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. the methods of sperm separation that have been repeatedly reported to isolate fractions rich in x- or y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. however, the ability of the se...

Abstract Over the past decades, Atlantic salmon ( Salmo salar, Salmonidae) has emerged as a model system for sexual maturation research, owing to high diversity of life history strategies, knowledge trait genetic architecture, and their economic value. The aim this synthesis is summarize current state concerning in salmon, outline gaps, provide roadmap future work. We knowledge: 1) takes place ...