This review summarizes the latest advances and main limitations for the implementation of in vitro embryo production programs in sheep and goats. We describe the laparoscopic assisted technique for oocyte retrieval and propose new insights for follicular manipulation to improve oocyte quality. Further description of the routine conducted in our laboratory for the in vitro process of oocyte matu...

As a process complementing conventional electrode reactions, ionic vacancy production in electrode reaction was theoretically examined; whether reaction is anodic or cathodic, based on the momentum conservation by Newton's second law of motion, electron transfer necessarily leads to the emission of original embryo vacancies, and dielectric polarization endows to them the same electric charge as...

This research was designed to evaluate the changes that might occur to protein production after nuclear transfer. Eight-cell stage pig embryo nuclei were transferred to enucleated metaphase II oocytes. Twelve hours after nuclear transfer the nuclear transfer embryos were labeled with L-[35S] methionine and subjected to 1-dimensional polyacrylamide gel electrophoresis. During normal pig embryo d...

Background: The aim of our study was to determine the metabolic profile: glucose, triglycerides (TG), high density lipoprotein (HDL) and cholesterol (CHT) in women undergoing ART (Assisted reproductive technology) cycles and estimate its effect on In Vitro Fertilization (IVF) outcomes Materials and Methods: Forty three women undergoing IVF treatment were included in the study. Patients were all...

A model was developed for the economic evaluation of embryos for producing bull lines for use in commercial beef production. The fundamental concept underlying the model is that a cloned and sexed embryo of known genetic characteristics for beef traits is used to produce a bull. After reaching physiological maturity, the bull is used in natural matings. Equations relating feed energy requiremen...

When cells are subjected to various stress factors, they increase the production of a group of proteins called heat shock proteins (hsp). Heat shock proteins are highly conserved proteins present in organisms ranging from bacteria to man. Heat shock proteins enable cells to survive adverse environmental conditions by preventing protein denaturation. Thus the physiological and pathological poten...

It is estimated that in the past five years, flow cytometric sorting of gender pre-selected sperm using DNA as the marker has produced over 30,000 offspring. The majority of these offspring were cattle for two reasons: 1) The cattle industry has accepted the use of gender specific sperm for commercial reproduction and 2) Cattle have a distinct advantage over swine in requiring a significantly l...

Embryo transfer in camels was initiated to respond to demand from the camel industry particularly in the United Arab Emirates since 1990. This paper reviews the research performed in critical areas of reproductive physiology and reproductive function evaluation that constitute a pre-requisite for a successful embryo transfer program. A description of donor and recipient management as well as a ...

Embryo quality during the in vitro developmental period is of great clinical importance. Experimental genetic studies during this period have demonstrated the association between specific gene expression profiles and the production of healthy blastocysts. Although the quality of the oocyte may play a major role in embryo development, it has been well established that the post - fertilization pe...

Systems for the in vitro production (IVP) of porcine embryos have advanced to the stage where large numbers of embryos can be reliably generated from the oocytes of abattoir-sourced ovaries in a single production run. These systems are extremely useful for studying various aspects of porcine gamete and embryo biology, developing associated reproductive technologies, such as embryo cryopreservat...