enzymatic extract preparation from pseudomonas aeruginosa km110 under accession no. hq730879 with lipase activity (0.3 u/ml), was used to perform enzymatic hydrolysis pretreatment of a synthetic dairy wastewater with 1000 mg/l total fat content. the pretreatment was optimized for 48 h hydrolysis time, at 45æ% c with 10% v/v enzymatic extract. the biological treatment of synthetic dairy wastewa...

a long-term study was conducted between 1985 and 2003 on rehabilitation trials at sishen ironore mine, south africa, to identify grass species that would survive in the artificial growth medium appliedto the sites, and to determine the most suitable medium for sustainable vegetation growth. vegetationestablishment was tested at slopes of 18° and 34° and with five different cover materials. afte...

gelatin is a protein hydrocolloid. because of its unique characteristics such as gel forming, thickening, stabilizing, emulsifiing, foam and film forming, is widely used in food industries. in this research, in order to optimize the factors which affect enzymatic extraction method of gelatin ,the effects of three variables, enzyme concentrations(2-10 ppm), time(8-16 hours) and temperature(60-80...

abstract          background and objectives: concentration low-density lipoprotein (ldl) is one of the strongest indicators of atherosclerosis and predicts the diagnosis of cardiovascular diseases. ldl measurement accuracy is very important. ldl can be measured directly, such as enzymatic and nephelometry methods or can be calculated using friedewald's formula. despite the development of e...

objective(s): irinotecan is a potent anti-cancer drug from camptothecin group which inhibits topoisomerase i. recently, biodegradable and biocompatible polymers such as poly lactide-co-glycolides (plga) have been considered for the preparation of nanoparticles (nps). materials and methods: in this study, irinotecan loaded plga nps were fabricated by an emulsification/solvent diffusion method to...

Internet databases of small molecules, their enzymatic reactions, and metabolism have emerged as useful tools in food science. Database searching is also introduced as part of chemistry or enzymology courses for food technology students. Such resources support the search for information about single compounds and facilitate the introduction of secondary analyses of large datasets. Information c...

ne of the important issues in molecular biology is the three-dimensional structure (shape) of proteins and deoxyribonucleic acid (DNA) in solution in the cell and the relationship between structure and function. Ordinarily, protein and DNA structure is determined by X-ray crystallography or electron microscopy. Because of the close packing needed for crystallization and the manipulation require...

A parallel approach to designing crystallization constructs for the c-FMS kinase domain was implemented, resulting in proteins suitable for structural studies. Sequence alignment and limited proteolysis were used to identify and eliminate unstructured and surface-exposed domains. A small library of chimeras was prepared in which the kinase insert domain of FMS was replaced with the kinase inser...

Hyaluronidase1 (HYAL1) is a hydrolytic enzyme that degrades hyaluronic acid (HA) and has three predicted N-glycosylation sites at Asn(99), Asn(216), and Asn(350). In this report, we show the functional significance of N-glycosylation on HYAL1 functions. Using mass spectrometry, we demonstrated that HYAL1 was N-glycosylated at the three asparagine residues. N-glycosylation of HYAL1 is important ...

Elucidating the relationship between the folding landscape of enzymes and their catalytic power has been one of the challenges of modern enzymology. The present work explores this issue by using a simplified folding model to generate the free-energy landscape of an enzyme and then to evaluate the activation barriers for the chemical step in different regions of the landscape. This approach is u...