The present work describes a comparative study involving two sequential injection enzymatic procedures for the spectrophotometric determination of glucose in milk samples. The determinations were based on the use of the enzymes, glucose oxidase and peroxidase, in solution or immobilized, and 4-aminophenazone and phenol as chromogenic reagents. In the first procedure, 8.4 IU of glucose oxidase a...

A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and...

We report here a new staining procedure which uses both the enzymatic dehydrogenation of 2-(p-iodophenyl)-3-p-(nitrophenyl)-5 phenyltetrazolium chloride to a pink intracellular formazan and the DNA-specific fluorochrome 4',6'-diamidino-2-phenylindole. Application of this staining procedure to cells concentrated on filters and then transferred to microscope slides by the filter-transfer-freeze t...

Aim: The study compares and evaluates enzymatic gel Brix 3000â Polymer bur (SS White) for caries excavation in primary molars terms of two parameters such as pain reaction the time taken excavation. Method: 60 children aged 7-9 years who require removal were recruited randomly allocated into groups: Group-A: Enzymatic (Brix 3000)â, Group-B: Smart burs White polymer burs). objectives evaluated w...

The article presents the results of research on influence enzymatic and oxidizing agents polymers used in drilling mud technology. calcium hypochlorite, urea peroxide, sodium persulfate, amylase cellulase. This allowed us to determine optimal concentrations these order reduce rheological parameters muds. ability enzymes oxidants decompose colloids composition muds was determined basis measureme...

Considerable variation in composition occurs among samples of ribonucleic acids from yeast. These variations are due in part to the procedures that are employed for isolation and subsequent purification. A recent study by Loring, Fairley, and Seagran (1) notes that variations may be due to (a) the occurrence of different ribonucleic acids in the particulate components of cells, (b) partial enzy...

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisation reaction. The single-stranded overhangs are designed to allow directional cloning in a ligase-free manner. This 'enzyme-free cloning'...

Enzymatic and homogeneous catalysis offer complementary means to produce enantiopure products. Incorporation of achiral, biotinylated aminodiphosphine-rhodium complexes in (strept)avidin affords enantioselective hydrogenation catalysts. A combined chemogenetic procedure allows the optimization of the activity and the selectivity of such artificial metalloenzymes: the reduction of acetamidoacryl...

We have developed an automated procedure on a discrete sample analyzer, Gilford System 3500, which alternately measures both total serum cholesterol and triglyceride concentrations as a "tandem" procedure. We used Dow Diagnostic's fully enzymatic, colorimetric reagents and aqueous standards to calculate unknowns ratiometrically. Cholesterol and then triglyceride reagent are dispensed into alter...