Amperometric biosensors consisting of oxidase and peroxidase have attracted great attention because of their wide application. The current work demonstrates a novel approach to construct an enzymatic biosensor based on TiO2 nanotube arrays (TiNTs) as a supporting electrode on which Prussian Blue (PB)-an "artificial enzyme peroxidase" and enzyme glucose oxidase (GOx) have been immobilized. For t...

The organic electrochemical transistor (OECT) can translate biochemical binding events between a recognition unit and its analyte into an electrical signal. We present OECT comprising n-type (electron transporting) conjugated polymer-based channel lateral gate electrode functionalized with the enzyme, glucose oxidase. device is integrated microfluidic system for real-time monitoring in flow-thr...

Materials Unless otherwise stated, FAD-dependent glucose dehydrogenase (E.C.: 1.1.99.10, Aspergillus sp., GLDE-70-1192) was purchased from Sekisui Diagnostics (UK) and used without any further purification. For comparison experiments, GDH was also purchased from Amano Enzyme Inc., Japan (“Amano 8”, Aspergillus oryzae) and BBI Solutions USA (GLD1, Aspergillus oryzae). Bilirubin oxidase (Myrothec...

The selective oxidation of the 6-hydroxy group of D-glucose to introduce an aldehyde functionality is not catalyzed by known oxidase enzymes. Selective functionalization at the glucose C-6 position in oligoand polysaccharides is a synthetically useful reaction that would greatly facilitate further chemical modifications for food, pharmaceutical, and materials applications. We chose the fungal e...

In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 microm diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-d...

A potentiometrically tuned-glucose biosensor was fabricated using core-shell nanocomposite based on zinc oxide encapsulated chitosan-graft-poly(vinyl alcohol) (ZnO/CHIT-g-PVAL). In a typical experiment, ZnO/CHIT-g-PVAL core-shell nanocomposite containing <20 nm ZnO nanoparticles was synthesized using wet-chemical method. The glucose responsive bio-electrode, i.e., glucose oxidase/ZnO/chitosan-g...

A method of tethering a mediator to an enzymatic membrane was studied to construct a non-leaking mediated glucose biosensor. Ferrocene carboxylic acid and glucose oxidase were immobilized in a sol gel derived silica (SGS) matrix containing cross-linked poly (vinyl alcohol) (CLPVA) and Nafion. CLPVA was applied as a solid support due to the ability to form very homogenous films with high quality...

Onion (Allium Cepa) contains high levels of flavonoids. Although there are many studies indicating the inhibitory effects of flavonoids on xanthine oxidase, there is no report on the effect of onion on this enzyme. Therefore, in the present study, the inhibitory effects of onion on xanthine oxidase are investigated. Fresh filtered juice of onion was prepared and its inhibitory effect on guine...

During neuronal communication neurotransmitters are released through the process exocytosis that occurs on the time scale of sub-milliseconds to milliseconds. Neuronal activity by neurotransmitters that are non-electroactive cannot easily be monitored due to limitations in the temporal resolutions of the sensor probes for these analytes. In order to achieve a fast detection of the rapid transie...

allopurinol, the xanthine oxidase inhibitor, is the only drug available for the treatment of gout. we examined the xanthine oxidase inhibitory activity of some commercially available flavonoids such asepigallocatechin, acacatechin, myricetin, naringenin, daidzein and glycitein by virtual screening and in-vitro studies. the interacting residues within the complex model and their contact types we...