We expect and encourage narrow performance ranges within-laboratory, since it is here that the greatest control over laboratory error is possible. This is well illustrated by Gilbert (2), where he indicates that about two-thirds of the inter-laboratory error is attributable to within-laboratory performance characteristics. We thank Dr. Bogdan for his letter and are grateful for the opportunity ...

We have evaluated four current Food and Drug Administration-cleared rapid tests for human immunodeficiency virus (HIV)-specific antibodies with a panel of specimens from recently infected individuals. Recent infection was detected by RNA-based screening coupled with enzyme immunoassay-based testing. We found that the sensitivities of the various rapid tests vary greatly with regard to their abi...

Background: Rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. Since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included Electron Microscopy (EM) and enzyme immunoassay. These methods, however, are expensive and not readily available everywhere. We have developed a technique which can be used for ...

We measured the concentrations of phenobarbital, phenytoin, primidone, ethosuximide, antipyrine, and caffeine in paired samples of saliva and plasma by gas chromatograph-mass spectrometer-computer (GC/MS/COM) and enzyme immunoassay. Mixed saliva was collected for the antipyrine and caffeine studies, parotid saliva for the phenobarbital, primidone, ethosuximide and phenytoin studies. The saliva/...

A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosida...

technique. This is almost certainly the case. 98-4%, respectively, for cervical specimens Towards the end of our reported study we and from 91-9% to 92-9% and 91-5% to noticed a decrease in the sensitivity of our 97-4% for urethral specimens, respectively. cell culture technique. The study was thereTaylor-Robinson, Hawkins, and fore extended, and discrepant results beThomas,1 cautioned against ...