The interaction of angiotensin converting enzyme (ACE) inhibition and atrial natriuretic factor (ANF) was investigated in six supine, sodium-replete, normal volunteers who received captopril (10 mg i.v. bolus followed by 10 mg/hr constant infusion) or vehicle superimposed on background 3-hour, constant, low-dose (1.5 pmol/kg/min) infusions of human ANF (99-126). Plasma converting enzyme activit...

During the course of work with kynureninase obtained from Neurospora cra~~cz (l), it became evident that a series of interactions was taking place between the enzyme and its substrates, pyridoxal phosphate and amines. Studies of the pyridoxal phosphate-activated dihydroxyphenylalanine and tyrosine decarboxylases have indicated that several amines (24) and aromatic amino acids (2, 5, 6) are capa...

During the course of work with kynureninase obtained from Neurospora cra~~cz (l), it became evident that a series of interactions was taking place between the enzyme and its substrates, pyridoxal phosphate and amines. Studies of the pyridoxal phosphate-activated dihydroxyphenylalanine and tyrosine decarboxylases have indicated that several amines (24) and aromatic amino acids (2, 5, 6) are capa...

Studies on the hydrodynamic properties of human milk bile salt-activated lipase (BAL) indicated that it is a monomer with molecular weight of 107,000. The presence of taurocholate (1 mM) did not lead to an association of the enzyme. The enzyme had a basal activity with trioctanoylglycerol and with shorter chain, but not with longer chain, monoacid triacylglycerols. Based on kinetic analyses, we...

It is important to correctly and efficiently predict the interaction of substrate-enzyme and to predict their product in metabolic pathway. In this work, a novel approach was introduced to encode substrate/product and enzyme molecules with molecular descriptors and physicochemical properties, respectively. Based on this encoding method, KNN was adopted to build the substrate-enzyme-product inte...

proper conditions for producing crude beta-galactosidase from waste materials were de-termined. this enzyme is to be used in the production of lactose-hydrolyzed milk. whey permeate was used as a basic medium. twenty seven treatments were developed by 3 vary-ing factors of: yeast extract (1, 2, and 3 %), wheat steep liquor (1, 2, and 3 %), and whey powder (0.5, 1, and 1.5 %). crude enzyme extra...

there are controversial reports about the effect of captopril on pain modulation. also while captopril may potentiate morphine analgesia, enalapril has not such an effect and interaction of morphine with angiotensin ii receptor antagonists and other angiotensin converting enzyme inhibitors has not been studied yet. therefore, this study was designed to assess the effect of captopril, lisinopril...

Understanding the physiological role of the plasma kallikrein-kinin system (KKS) has been hampered by not knowing how the proteins of this proteolytic system, when assembled in the intravascular compartment, become activated under physiological conditions. Recent studies indicate that the enzyme prolylcarboxypeptidase, an ANG II inactivating enzyme, is a prekallikrein activator. The ability of ...

this study investigated the influence of inoculation with an arbuscular mycorrhizal fungus (amf), glomus mosseae (nicol & gerd.) on growth and antioxidant enzyme activities (sod, cat, gupx) in shoots and roots of wheat (triticum aestivum l.) affected by three different levels of salt stress. the experiment was arranged as a factorial in randomized complete block design (rcbd) with three replicat...

The main components of biosensors are based on well-understood physical processes (such as diffusion, convective flow, energy and mass transfer) as well as chemical and biological reactions, all of which are amenable to differential equations. Using mathematical and computational modeling techniques to characterize the biosensor response as a function of its input parameter in wide range of phy...