conclusions high hev seroprevalence (16.7%) was observed among the blood donors in bushehr province. it appears that exposure to hev increases with age; although, more people should be examined. results overall, 105 (16.7%) blood samples were positive for hev-specific-igg antibodies, while 523 (83.8%) were negative. the presence of anti-hev igg was not associated with gender; however, it was co...

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease. the staphylococcal superantigens are considered as the causative agent of ra disease. objectives this study aimed to assess the presence of staphylococcal enterotoxin d in synovial fluid and blood of patients with ra. patients and methods a total of 120 blood and sf samples of patients with ra were studied. ba...

background the etiology of the guillain-barre syndrome (gbs) is not clear; in many cases, gbs is triggered by a bacterial or viral illness. objectives to evaluate the role of active infection with herpesviridae group and its association with this syndrome we have investigated the cerebrospinal fluid (csf) and serum of patients with gbs for detection of these viruses. patients and methods in a pr...

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

This study aims to determine of Chloramphenicol test for Vannamei shrimp conducted ELISA (Enzyme-Linked Immunosorbent Assay) method with 1 treatment. is done by adding the Abenzyme conjugate, and ends addition substrate reaction stop buffer. Each treatment was performed three times. The value (CAP) (Litopenaeus vannamei) which tested at 4 weeks ranged from 0.021 0.153 ppb. result showed in stil...

Hakkari ili domates ve biber üretim alanlarında lekeli solgunluk virüsü (Tomato spotted wilt virus, TSWV) yonca mozaik (Alfalfa mosaic AMV)’nün belirlenmesi için yapılan sürveyler 2020 döneminde gerçekleştirilmiştir. Domates sararma, nekroz, şekil bozukluğu simptomu gösteren 184 bitkiden yaprak örneği toplanmıştır. Toplanan örneklerinde TSWV AMV’nin Double antibody sandwich enzyme linked immuno...

results we found 28 cases with positive results for b. melitensis by multiplex pcr technique which was significantly different from of sat (p<0.05). six samples were positive for b. abortus by pcr. conclusion the results of present study showed that multiplex pcr assay is a rapid and sensitive technique for diagnosis of brucellosis compared to sat. however it is more accurate when coupled with ...

conclusions the present findings reveal a relatively high presence of anti-hev iggs and clearly indicate that hev infection is still present in northern algeria. further, the prediction of hev genotype using different antigens generated from the different hev genotypes shows that the causative strains are more likely to be of genotype 1. background two hepatitis e virus (hev) outbreaks occurred...

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease of unknown etiology. in this regard, the role of bacterial superantigens (as an effective agent) were considered. objectives this study aimed to assess staphylococcal enterotoxin e in the blood of patients with rheumatoid arthritis. patients and methods a total of 83 blood samples of patients with ra were studi...

background: nowadays, chlamydia trachomatis is known as a causative agent of infertility. because of, asymptomatic nature of infection, many may suffer from its lasting complications such as infertility. this study was performed in tehran during april 2007 to april 2008 to compare the prevalence of chlamydia trachomatis infection in fertile and infertile women using elisa and pcr methods. metho...