To immobilize enzymes at the surface of a nanoparticle-based electrochemical sensor is a common method to construct biosensors for non-electroactive analytes. Studying the interactions between the enzymes and nanoparticle support is of great importance in optimizing the conditions for biosensor design. This can be achieved by using a combination of analytical methods to carefully characterize t...

This paper presents a biosensor-CMOS platform for measuring the capacitive coupling of biorecognition elements. The biosensor is designed, fabricated, and tested for the detection and quantification of a protein that reveals the presence of early-stage cancer. For the first time, the spermidine/spermine N1 acetyltransferase (SSAT) enzyme has been screened and quantified on the surface of a capa...

In this work, cross-sensitivities and environmental influences on the sensitivityand the functionality of an enzyme-based amperometric sensor system for the directdetection of formaldehyde from the gas phase are studied. The sensor shows a linearresponse curve for formaldehyde in the tested range (0 - 15 vppm) with a sensitivity of1.9 μA/ppm and a detection limit of about 130 ppb. Cross-sensiti...

We describe an inkjet printed paper-based sensor that reports ATP by the enzyme catalysed hydrolysis of S-methyl-L-cysteine generating an odour (methyl mercaptan) that is easily detectable by the human nose.

We report an enzymatic sensor based on an organic electrochemical transistor that uses a room temperature ionic liquid as an integral part of its structure and as an immobilization medium for the enzyme and the mediator.

Protein immobilization on solid interfaces is a crucial aspect of their successful application in technologies such as biosensing, purification, separation, decontamination, etc. Although immobilization can improve the long-term and operational stability of proteins, this is often at the cost of significant losses in the catalytic activity of the tethered enzyme. Covalent attachment methods tak...

Abstract Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET required to elucidate its in such processes. Ultrafast VIS‐pump/IR‐probe spectroscopy can detect pathways proteins. However, the requirement having a donor sensor installed simultan...