Enzymes are the most proficient catalysts, offering much more competitive processes compared to chemical catalysts. The number of industrial applications for enzymes has exploded in recent years, mainly owing to advances in protein engineering technology and environmental and economic necessities. Herein, we review recent progress in enzyme biocatalysis, and discuss the trends and strategies th...

Lysolecithin is preferentially used in food technology, in animal feed products, and in cosmetics and pharmaceutical preparations as a surfactant.i} Industrially, lysolecithin s produced by enzymatic hydrolysis of Iecithin, The most eommon enzyme used is pancreatin prepared from porcine pancreas. However, the use of pancreatin has certain disadvantages, such as its limited supply, its high cost...

AN ACTIVE ENZYME CONSTRUCTED FROM A NINE AMINO ACID ALPHABET Kai U. Walter, Katherina Vamvaca and Donald Hilvert From the Laboratory of Organic Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zürich, Switzerland Running title: 9 amino acid enzyme Address correspondence to: Donald Hilvert, Laboratorium für Organische Chemie, ETH Hönggerberg/ HCI F 339, CH-8093 Zürich, ...

This review paper aimed to provides precious information about the function and use of different enzymes in dairy food applications. An enzyme is called a protein and catalyzes a specific reaction. Every enzyme is intended to initiate a particular reaction with a specific outcome. Moreover, numerous enzymes are present in the human body. Dairy food applications include the use of different enzy...

Biocatalysis is a useful tool in the provision of chiral technology and extremophilic enzymes are just one component in that toolbox. Their role is not always attributable to their extremophilic properties; as with any biocatalyst certain other criteria should be satisfied. Those requirements for a useful biocatalyst will be discussed including issues of selectivity, volume efficiency, security...

Immobilization technology creates exciting new opportunities for commercial development in a wide range of industries. There are several reasons for using an enzyme in an immobilized form. In addition to more convenient handling of the enzyme, it provides for its facile separation from the product, thereby minimizing or eliminating protein contamination of the product. To date, pectinases have ...

This paper gives an overview of the literature data concerning specific and non specific inhibitors of Na⁺,K⁺-ATPase receptor. The immobilization approaches developed to improve the rather low time and temperature stability of Na⁺,K⁺-ATPase, as well to preserve the enzyme properties were overviewed. The functional immobilization of Na⁺,K⁺-ATPase receptor as the target, with preservation of the ...

An efficient and rapid affinity-based screening method for directly fishing out natural alpha-glucosidase inhibitors from Cyperus. rotundus extract by using immobilized enzyme technology combined with UHPLC-QTOF MS analysis was established. As a results, without time-consuming and laborious isolation workload and false positive interference, five natural alpha-glucosidase inhibitors were succes...

laboratory diagnosis of acute measles is usually achieved by serology assays for measle-specific igm antibody. for comparison of measle-specific igm antibody in saliva and serum, 95 paired blood and saliva samples were collected 1-14 days after the onset of rash. the specimens were tested for specific igm antibody by an igm antibody-capture enzyme immunoassay (eia). measles igm antibody was det...

the aim of this study was to evaluate an enzyme - linked immunosorbent assay (elisa) for the detection of anti - helicobacter pylori (h. pylori) specific igg antibodies in specimens of oral fluid. all subjects over the age 18 years undergoing endoscopy for any reason were asked to participate in the study. two groups of 44 patients in each were selected as hp+ and hp-. at the same time, 5 milli...