Previous studies with phospholipid monolayers revealed a large decrease in the activity of phosphoinositide-specific phospholipase C-delta(1) (PLC-delta(1)) which catalyses the hydrolysis of PtdIns(4, 5)P(2) as lateral pressure is applied to the membrane. If stress on the membrane is the sole inhibitor of PLC-delta(1) activity, the enzyme must penetrate the membrane surface to engage its substr...

Quantitative effects of altering oxidative phosphorylation and respiration on the activity of the enzyme menadione reductase (NAD[P]H,:2-methyl-i,4-naphthoquinone oxidoreductase, E.C. 1.6.5.2), in stabilized polymorphonuclear neutrophil leucocytes of Amphiuma tridactylum, were studied by amplitude-contrast microscopy and microspectrophotometry. In a general way, the rate of enzymic activity was...

Alpha-crystallin is a member of the small heat-shock protein family and functions like a molecular chaperone, and may thus help in maintaining the transparency of the eye lens by protecting the lens proteins from various stress conditions. Non-enzymic glycation of long-lived proteins has been implicated in several age- and diabetes-related complications, including cataract. Dicarbonyl compounds...

BACKGROUND Individuals with low activity of a key metabolic enzyme, thiopurine methyl transferase (TPMT), are more susceptible to azathioprine-induced myelosuppression. AIM To determine the pattern of use of TPMT activity estimation, with respect to azathioprine use, by medical practitioners in the UK. DESIGN Retrospective analysis of assay use. METHODS We analysed all test results (n=329...