BACKGROUND AND PURPOSE Essential oils extracted from different plants are extensively used in perfume, beverage, and food industries and are reported to exhibit antimicrobial activities against a variety of fungi. Zhumeria majdae belonging to the Lamiaceae family is a rare and endemic medicinal plant species in Iran, with a strong and pleasant odor. The leaves of this plant have been used for m...

An oligonucleotide microarray based on the arrayed-primer extension (APEX) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. Species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (ITS1 and ITS2) region were designed for 24 species belonging to 10 genera, including Cand...

Intra- and interspecies variations of the translation elongation factor 1-α (Tef-1α) gene were evaluated as a new identification marker in a wide range of dermatophytes, which included 167 strains of 30 species. An optimized pan-dermatophyte primer pair was designed, and the target was sequenced. Consensus sequences were used for multiple alignment and phylogenetic tree analysis and the levels ...

27 28 An oligonucleotide microarray, based on the arrayed primer extension (APEX) technique, has been 29 developed to simultaneously identify pathogenic fungi frequently isolated from invasive and 30 superficial infections. Species-specific oligonuceotide probes complementary to the internal 31 transcribed spacer (ITS1 and ITS2) region were designed for 24 species belonging to 10 genera, 32 inc...

A new mycological medium, Fishmeal Extract Dextrose Agar (FEDA) was developed using aqueous extract of fishmeal as a substitute for peptone and evaluated for its efficacy in supporting the growth of fungi commonly encountered in clinical specimens and in maintaining fungal stock cultures. Trichophyton rubrum, Trichophyton mentagrophyte, Microsporum gypsium, Microsporum nanum, Epidermophyton flo...

Skin disease associated with keratinized tissues in animal and human beings has been investigated. The essential oil of Eucalyptus pauciflora in vitro showed strong antifungal activity at 1.0 microl/ml against human pathogenic fungi, viz. Epidermophyton floccosum, Microsporum audouinii, M. canis, M. gypseum, M. nanum, Trichophyton mentagrophytes, T. rubrum, T. tonsurans and T. violaceum. The oi...

This study examined the frequency of dermatophytoses in the Primorsko-Goranska County, a north-western part of Croatia, over a period of 21 years (1988-2008). All fungal samples were microscopically examined with 20% potassium hydroxide (KOH) solution. Fungal infections were confirmed in 26.9% cases. Out of these, dermatophytes were isolated in 38.3%, Candida spp. infection in 55.1% cases, whil...

Background: Dermatophytosis was a fungal infection caused by molds (dermatophytes). Dermatophytes are group of closely related filamentous fungi able to damage and utilize keratin found in the skin, hair, nails. Objective: To isolate species which cause resistant dermatophytosis, clinical types tinea, as well assess response different antifungal therapies.
 Patients Methods: Ninety-two pat...

A rapid two-step DNA extraction method and a multiplex PCR for the detection of dermatophytes in general and Trichophyton rubrum specifically were developed and evaluated with DNA extracted from pure cultures and from clinically diseased nails. DNA from the following dermatophytes was used: Epidermophyton floccosum, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Microsporum nanu...