background: the refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. objectives: the purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produc...

purpose : to evaluate the effect of topical diclofenac sodium 0.1% on the corneal epithelial healing after photorefractive keratectomy (prk) methods : in this prospective randomized double blind clinical trial, prk was performed on 82 patients. thirty-three cases of them receiving topical diclofenac four times per day after surgical procedure and 45 patients did not receive this medication as a...

background: endometriosis is a complex estrogen-dependent disease that is defined as the presence of endometrial gland and stroma outside the uterine cavity. although the exact mechanism for the development of endometriosis remains unclear, there is a large body of research data and circumstantial evidence that suggests a crucial role of estrogen in the establishment and maintenance of this dis...

Secretory meningioma have been described as a distinct variant of meningioma based on their histologic, immunohistochemical and ultrastructural features of epithelial and secretory differentiation of meningothelial cells with accumulation of secretory material in the form of hyaline inclusion. Secretory meningioma is also a benign tumor having similar biological behaviour to that of typical men...

The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stain...

AIMS Recent studies suggest that paediatric renal cell carcinoma (RCC) may represent a distinct group of tumours; however, its biological behaviour and classification remain poorly understood. The aim was to analyse 13 RCCs from patients < or =23 years of age to determine their clinicopathological, immunohistochemical and molecular characteristics. METHODS AND RESULTS The histological spectru...

amniotic epithelial cells (aecs) possess unique characteristics, which make them a suitable source for cell-based therapeutic strategies. aecs have stem cell properties with low-immunogenicity (due to expressing hla-g molecule and absence of mhc class i and ii antigens) and no ethical problems, as well as availability in sufficient numbers, which can be obtained from a placenta. we have recentl...

Objective: To determine whether platelet-lymphocyte ratio can be a prognostic factor for epithelial ovarian cancer. Methods: This study was retrospective cohort with analytical design, conducted in the Department of Obstetrics and Gynecology Prof. Dr. R.D. Kandou Manado General Hospital from January – November 2020. The subjects were all patients cancer who met inclusion exclusion criteria. Dat...

A 15-year-old Asian male, with a history of skin allergy and asthma, presented complaints itching, photophobia, redness in both eyes for the last 10 years. His right eye showed upper lid papillae, perilimbal conjunctival pigmentation, gelatinous limbal hyperplasia temporal quadrant (Fig A), pseudogerontoxon involving nasal cornea B). The superior limbus multiple raised, circumscribed, transpare...

Human airway epithelial cells can increase expression of both lactoferrin and ferritin after exposure to catalytically active metal. These proteins transport and store metal, with coordination sites fully complexed, and therefore can diminish the oxidative stress. The intracellular transport of lactoferrin results in a transfer of complexed metal to ferritin, where it is stored in a less reacti...