The last decade was noted for the emergence and prevalence of antimicrobial agent-resistant pneumococci. Strains resistant to macrolides, chloramphenicol, and tetracycline have been registered in a number of countries (1). In some regions, resistance to macrolides prevails over the resistance to penicillin. Macrolide resistance has been reported to be high among pneumococci in Asian countries (...

The bactericidal activities of ABT-773, a new ketolide, were compared to those of cefuroxime and amoxicillin-clavulanate against 10 strains of Streptococcus pneumoniae containing the ermB gene. MICs and time-kill curves were determined in duplicate per NCCLS guidelines with cation-adjusted Mueller-Hinton broth with 3% lysed horse blood. Viable counts were done at 0, 2, 6, and 24 h. Antibiotic c...

Using Enterococcus faecium strains as probiotics raises several controversies related to their antibiotic resistance (AR). In the current study, we examined isolates of E. obtained from human breast milk. Catalase-negative and γ-haemolytic were identified by analyzing sequences 16S rRNA gene phenotypic antibiotics was investigated. We expression genes that found on plasmids. The majority tested...

Abstract The aim of this study was to investigate the rate resistance macrolide-lincosamide-streptogramin B (MLSB) antibiotics, mechanisms underlying and evaluate their relationship with virulence genes profiles 435 Bulgarian clinical isolates Staphylococcus aureus . highest observed penicillin (96.09%), followed by erythromycin clindamycin (34.02 22.76%, respectively). Of tested strains S. , 9...

Enterococcus is a bacterial genus that strongly associated with nosocomial infections and has high capacity to transfer acquire resistance genes. In this study, the main objective was evaluate presence of species in ornamental animal feed characterize their antimicrobial virulence factors. Antimicrobial susceptibility determined using 14 agents by disk diffusion method, complemented genotypic a...

ABSTRACT: Enterococci have been used as sentinel organisms for monitoring antimicrobial resistance in food, humans, and other animals. In this sense, the present study evaluated susceptibility profile presence of genes associated with to erythromycin (msrC ermB) tetracycline [tet(M) and/or tet(L)] enterococci isolated from raw sheep’s milk cheeses (colonial, feta-, pecorino-type) South region B...

To identify and map genes involved in competence for genetic transformation in Streptococcus pneumoniae, we have cloned DNA surrounding an ermB insertion mutation that causes a competence factor deficiency. We recovered the insert and approximately 500 base pairs of neighboring pneumococcal DNA in pMB9. Larger pieces of DNA from this region were unstable in pMB9 and pBR325. However, larger piec...

Erythromycin resistance rates among penicillin-susceptible Streptococcus pneumoniae were 38 and 92% among penicillin-intermediate and -resistant S. pneumoniae isolates from Hong Kong, respectively, and 27% (43 of 158) of the isolates showed the MLS(B) phenotype, and the majority carried the ermB gene; 73% (115 of 158) displayed the M phenotype, and all possessed the mef gene. The MLS(B) phenoty...

Erythromycin resistance among streptococci is commonly due to target site modification by an rRNA-methylating enzyme, which results in coresistance to macrolide, lincosamide, and streptogramin B antibiotics (MLSB resistance). Genes belonging to the ermAM (ermB) gene class are the only erythromycin resistance methylase (erm) genes in Streptococcus pyogenes with MLSB resistance that have been seq...