The gene pelA encoding a pectate lyase from the strain Bacillus sp. BP-23 was cloned and expressed in Escherichia coli. The nucleotide sequence of a 1214 bp DNA fragment containing pelA gene was determined, revealing an ORF of 666 nucleotides that encoded a protein of 23233 Da. The deduced amino acid sequence of the encoded enzyme showed homology to pectate lyases A, B, C and D from Fusarium so...

L-asparaginase is an enzyme that catalyzes the conversion of L-asparagine to L-aspartate and ammonia. The important application of the L-asparaginase enzyme is in the treatment of acute lymphoblastic leukemia, Hodgkin disease, acute myelocytic leukemia, chronic lymphocytic leukemia, lymphosarcoma treatment, reticulosarcoma and melanosarcoma. In the present study, the L-asparaginase from Erwinia...

Magainin peptides originally identified from Xenopus laevis have cytotoxic effects against a range of prokaryotic organisms without harmful effects on higher eukaryotes. The mechanism of cytotoxicity of the peptides is by disruption of membranes, which causes osmolysis. Magainin peptides are known to inhibit the in vitro growth of many phytopathogens including Erwinia carotovora, the causative ...