background and objectives: the most common serotype of enterohaemorrhagic esherichia coli group or shiga-toxin-producing e. coli is o157:h7. domestic and wild ruminants are regarded as the main natural reservoirs. o157:h7 serotype is the major cause of gastrointestinal infections in developed countries. in this study was conducted to survey on the toxigenic e. coli o157: h7 strains in milk of i...

background and objectives: auto-induction is usually employed to achieve high cell density and overproduction of proteins with a simple and low-cost operation. the efficiency of heterologous protein expression in escherichia coli is determined by different parameters. interactions between these parameters usually complicate the identification of those that contribute more to the improvement of ...

background escherichia coli (e. coli) species are able to produce extended-spectrum β-lactamases (esbls) that cause high resistance to the beta-lactam antibiotics. therefore, determining the antibiotic susceptibility patterns in resistant organisms is necessary for suitable therapeutic approaches. patients and methods totally, 260 clinical isolates of e. coli were collected from hospitals in te...

abstract        background and objective: the formation of urinary infection by uropathogenic e.coli needs   numerous virulence factors and biofilm formation is among these factors. bacteria that form biofilms express phenotype traits that appear according to the bacteria type. cellulose is an important compound on the outside of e.coli causing bacterial cell-cell reactions and connection to no...

phylogenetic analysis has shown that escherichia coli is composed of four main phylogenetic groups (a, b1, b2 and d). characterization of phylogenetic groups is of clinical interest, as group a and b1 generally associated with commensals, whereas most enteropathogenic isolates are assigned to group d, and group b2 is associated with extra-intestinal pathotype. one hundred e. coli strains recove...

background: although enteral feeding solutions are used to reduce the morbidity and mortality in many malnourished and hospitalized patients, microbial contamination of these products may lead to severe infections, especially in immune suppressed ones. the aim of this study was to evaluate the microbiological quality of commercial enteral feedings in two hospital settings in shiraz. methods: tw...

today, it is nearly 25 years past from investigation of shiga toxigenic escherichia coli (stec) which is able to produce shiga toxins and cause different gastroenteritis. since incidence of gastroenteritis due to stec is increasing, it's necessary to develop rapid, specific and accurate procedures like pcr. in this study, we used pcr method to detect and identify stec in cultures of 55 escheric...

cloning and sequencing of a lipase gene from an indigenous bacillus pumilus, strain f3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. sequence analysis showed that f3 lipase contained a signal peptide composed of 34 amino acids with an h domain of 18 residues. a tat-like motif was found in the signal peptide similar to some other bacillus pumil...

Fimbrial adhesion is a Virulence Determinant which is classified under Adhesins category of virulence factor of uropathogenic Escherichia coli. Afimbrial adhesin Protein with swissprot accession number P12730, of length 181 amino was selected for modeling using Bioinformatics tools. Modelled protein has been submitted to protein model database and has been assigned an accession number of PM0075...

Abdoul-Salam Ouédraogo, Fabrice Compain, Mahamoudou Sanou, Salim Aberkane, Nicolas Bouzinbi, Mallorie Hide, Lassana Sangaré, Rasmata Ouédraogo-Traoré, Hélène Jean-Pierre, Julie Vendrell, Jérôme Solassol, Dominique Decré, Sylvain Godreuil Département de bactériologie-virologie, Centre hospitalier régional universitaire (CHRU) de Montpellier, Montpellier, France; Université de Montpellier, Montpe...