The structural characterization of proteins using electrospray ionization mass spectrometry (ESI-MS) has become an important method for understanding protein structural dynamics. The correlation between the structures of proteins in solution and gas phase needs to be understood for the application of ESI-MS to protein structural studies. Hen egg white lysozyme (Lyz) is a small protein with a st...

INTRODUCTION Therapy with fixed orthodontic appliances starts with bracket bonding and ends with debonding of brackets, leaving enamel surface varied. Objective The aim of this pilot study was to examine enamel surface before and after debonding of orthodontic brackets by the use of scanning electron microscopy (SEM). METHODS Epoxy replicas of four patients' premolars indicated for therapy wi...

BackgroundCoarctation of the aorta (CoA) is often treated percutaneously. The aim this study was to describe immediate results percutaneous management native aortic coarctation (NaCoA) and recoarctation (ReCoA) at our institution.MethodsWe identified all patients with NaCoA or ReCoA who underwent dilatation by either balloon angioplasty (BAP) endovascular stent implantation (ESI) between 2011 2...

A prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. A total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were re...

Two peptide-oligothymidylic acids, prepared by joining an 11 residue synthetic peptide containing one internal carboxyl group (Asp side chain) to amino-linker-5'pdT6 and amino-linker-5'pdT10 oligonucleotides, were analyzed by matrix-assisted laser desorption/ionization (MALDI) on a linear time-of-flight mass spectrometer and by electrospray ionization (ESI) on a triple-quadrupole system. These ...

For the period January 2005 to June 2008, we reviewed the rates, causes, and outcomes of exit-site infection (ESI) among 137 consecutive patients [mean age: 51 +/- 16 years; 17 (12.4%) with diabetes; 76 (55%) on automated PD; time at risk: 240.41 dialysis years; mean follow-up: 20.4 +/- 13.8 months]. Treatment protocol included mini-laparotomy and Popovich-Moncrief placement method, with presur...

Electrochemistry (EC) is a mature scientific discipline aimed to study the movement of electrons in an oxidation-reduction reaction. EC covers techniques that use a measurement of potential, charge, or current to determine the concentration or the chemical reactivity of analytes. The electrical signal is directly converted into chemical information. For in-depth characterization of complex elec...

When John Fenn and coworkers first realized Malcolm Dole's dream of electrospray ionization (ESI) (1), the broad impact it would ultimately have was not obvious to most researchers working in the (admittedly much smaller) field at the time (2). Indeed, between his first publication in 1984 and ground breaking report in 1988 that described ESI-mass spectrometry (ESI-MS) as a tool for analyzing l...

The response of many previously low-detectable or undetectable compounds in electrospray ionization mass spectrometry (ESI-MS) has been enhanced by the addition of a simple, homemade needle into the traditional ESI interface. The needle located between the ESI emitter and the ion sweep cone (inlet of the detector) would ionize those neutral gaseous compounds, formed during electrospray, by a co...

Results: During this period, a total of 376 Tenckhoff catheters were implanted and 312 patients were treated. In 34.5% of the patients transferred to haemodialysis the cause was access related infection. Global catheter cumulative survival was 82%, 64% and 50% at 1, 3, and 5 years respectively. A total of 35 subcutaneous cuff excisions were electively performed in 33 patients (one patient had c...