DeFries, Christa, "Regulation of levels and localization of thimet oligopeptidase in prostate cancer cells by β-estradiol" (2013). Honors Thesis Collection. Paper 161. Abstract The enzyme thimet oligopeptidease (TOP) catalyzes the hydrolysis of gonadotropin-releasing hormone (GnRH) in vitro, and evidence suggests that TOP is the primary enzyme responsible for GnRH hydrolysis in vivo, as well. I...

OSTEOPOROSIS IS A MAJOR cause of disability and excess mortality in older women. Estrogen therapy has been used for treatment and prevention of osteoporosis; however, many older women are reluctant to use it because of adverse effects. Recent data from the Women’s Health Initiative demonstrated that postmenopausal women who took hormone therapy for approximately 7 years had decreased hip fractu...

BACKGROUND The endometrium becomes receptive to the embryo after sequential actions of estrogen and progesterone. The purpose of this study was to examine the effects of estrogen and progesterone on endometrial hemodynamics and on secretion of vascular endothelial growth factor (VEGF) from endometrial epithelial cells (EEC). METHODS Six early postmenopausal women taking sequential estrogen an...

A subclone of rat pituitary tumor cells, designated GH3/C14, was isolated from the parent population of GH3 pituitary cells and was estrogen-dependent for growth in vivo. GH3/C14 cells inoculated into host animals formed tumors in intact females, estrogen-treated ovariectomized females, and estrogen-treated males, but not in untreated intact or castrated males, or untreated ovariectomized femal...

Genome-wide association studies (GWAS) have successfully identified common genetic variants that contribute to breast cancer risk. Discovering additional variants has become difficult, as power to detect variants of weaker effect with present sample sizes is limited. An alternative approach is to look for variants associated with quantitative traits that in turn affect disease risk. As exposure...

The endocrine status of 49 premenopausal women taking tamoxifen after completion of adjuvant chemotherapy for breast cancer was determined by radioimmunoassay from serial blood samples. Of these 49 women, 7 had regular menses, 14 had irregular menses, 23 were amenorrheic, and 5 had undergone hysterectomies. A group of 12 premenopausal women who had no history of breast cancer and were not takin...

Plasma concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL) were determined over a 24-h period using radioimmunoassay in sheep injected with corn oil (control) or various doses of zeranol or estradiol-17 beta. Injection of .333, 1 or 10 mg of zeranol caused dose-related increases (P less than .01) in plasma PRL (peak levels at 12 to 18 h) and LH (pe...

brain edema plays an important role in secondary tissue damage following traumatic brain injury (tbi) but the underlying mechanisms are not entirely elucidated. the g protein coupled receptor fm-4 and its ligands, neuromedin s (nms) and neuromedin u (nmu), are expressed in diverse brain areas, and have a variety of roles in nociception, inflammation, and stress but their probable changes after ...

The impact of steroid hormones, and particularly estradiol, on human microbiota could be recognized as a substantial part human-microbiota interactions. However, an area that remains poorly investigated is the skin vaginal microbial communities biofilms, which contain non-pathogenic bacteria phyla Firmicutes Actinobacteria, especially probiotic genus Lactobacillus widespread, safe genus, Microc...

Introduction and Historical Background ........................................... 2 Hormone Metabolism and Toxicity in Humans ................................ 4 Toxicity Studies in Animals and Cell Cultures ................................. 6 Genotoxicity Assays ........................................................ 6 Carcinogenicity Assays ......................................................