Single pollen grain polymerase chain reaction (PCR) has succeeded in several species, however only limited numbers of pollen grains were involved due to difficulties in pollen isolation and lysis. This has limited its application in genetic analysis and mapping studies in plants. A high-throughput (HT) procedure for collecting and detecting genetic variation in a large number of individual poll...

Preparations of pure macrophage suspensions may be required in the investigation of cellmediated immune reactions or for the preparation of antimacrophagic serum. Methods for the preparation of macrophage suspensions of high purity from peritoneal exudates are relatively tedious and result in small yields of cells. Bloom and Bennett (1966) and Turk and Polak (1967) describe a technique inwhich ...

Background and objectives: .The purpose of this study was to evaluate the effect of smear layer removal on apical seal and the quantity of microleakage associated with lateral cold condensation and thermafil obturation. Methods: Forty extracted single–rooted human teeth were randomly divided into two experimental groups; in group A, 20 roots with smear layer free, 5.25 % sodium hypochlorite (Na...

Adherence of chicken heterophils was evaluated at 37 C using preconstructed columns containing various weights of nylon fiber (75 mg, 100 mg, or 125 mg) and whole blood anticoagulated with sodium heparin or 10% disodium ethylenediamine tetraacetic acid (EDTA). Additionally, 50-mg and 75-mg nylon fiber columns incubated at 41 C were used to evaluate heterophil adherence at an increased temperatu...

An optimized extraction and cleanup method for the analysis of chlortetracycline (CTC), doxycycline (DC), oxytetracycline (OTC) and tetracycline (TC) in soil is presented. Soil extraction in a pressurized liquid extraction system, followed by extract clean up using solid-phase extraction (SPE) and tetracycline determination by liquid chromatography tandem mass spectrometry (LC-MS/MS) provided a...

OBJECTIVES This study compared the ability of several techniques to remove calcium hydroxide (CH) from the root canal and determined the influence of CH residues on the accuracy of the electronic apex locator. MATERIALS AND METHODS Root canals of 90 human maxillary lateral incisors with confirmed true working length (TWL) were prepared and filled with CH. The teeth were randomly assigned to o...

AIM The present study was conducted to know the ultrastructural detail of the blood cells of Uttara fowl (native fowl of Uttarakhand). MATERIALS AND METHODS The experiment was conducted on 10 apparently healthy adult birds of either sex reared at the Instructional Poultry Farm, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand. The blood was collected from wing vein u...

Oligomers containing pendant isocyanate groups were synthesized from various vinyl monomers, m-isopropenyldimethylbenzyl isocyanate (TMI), and 2-isocyanatoethyl methacrylate (IEM). The liquids were characterized by their refractive indices, infrared spectra, and percentage of isocynate groups in the molecule. Adhesive properties of these compounds were compared with those of oligomers prepared ...

The distribution of rare earth elements (REE) in a pooled soil sample collected from Zhangzhou, Fujian Province, China, was screened by a five-step sequential extraction procedure coupled with ICP-MS determination after preconcentration of REE and removal of the matrix by extraction with 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (HPMBP). The results showed that the distribution of REE in the dif...

We compared the performance of an in-house and a commercial malaria polymerase chain reaction (PCR) assay using freeze-thawed hemolytic blood samples. A total of 116 freeze-thawed ethylenediamine tetraacetic acid (EDTA) blood samples of patients with suspicion of malaria were analyzed by an in-house as well as by a commercially available real-time PCR. Concordant malaria negative PCR results we...