نتایج جستجو برای: expected heterozygosity
تعداد نتایج: 255230 فیلتر نتایج به سال:
Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 a...
Nuclear markers such as microsatellites have allowed the identification of conservation and management populations of the Hawksbill turtles. In present study, eight microsatellite loci were studied. 60 samples of hawksbill turtles. flipper from Shidvar and Hormuz Islands have been surgically removed and preserved in 20% DMSO buffer. DNA was extracted using DNP KIT and amplified by PCR methods. ...
We report on a newborn with IUGR, rhizomelic dwarfism, and suspected chondrodysplasia punctata. At birth, OI was suspected; however, a skeletal survey suggested ML II alpha/beta. Sequencing revealed compound heterozygosity for a reported pathogenic and novel but expected pathogenic GNPTAB variant. Molecular testing for autosomal recessive OI identified a SERPINF1 variant.
the polymorphism in exon three of fabp4 gene and its association with growth traits of 45 sistani (n=30) and dashtiari (n=15) cattle were investigated. dna extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. animal genotypes were determined based on polymerase chain reaction (pcr) products and their band size electrophorese...
objective: classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. molecular cytogenetic techniques have also recently been developed and used for this purpose. quantitative florescence pcr using short tandem repeat (str) markers has more potential for high throughput diagnosis. marker heterozygosity in short tandem repeats (str) is ...
mahi sefid (cyprinidae) is an economically important fish in the south caspian sea. specimens was collected from the lamir river, sefid rud, shir rud and tajan rivers and analyzed at 38 microsatellite dna loci. eight primers out of the thirty analyzed loci were polymorphic in all sampling regions. the highest observed heterozygosity (1) was in locus ca1 in sefid rud and lowest was 0.07 in locus...
this study was conducted to investigate the single nucleotide polymorphism of stat1 gene by pcr-rflp technique and association of genotypes with milk production traits in brown swiss dairy cattle. the blood samples of ninety eight cows were collected randomly from tail vein. the dna was extracted with cinagene kit (pr881612c). fragment of 314 bp of stat1 gene was amplified by pcr and digested b...
the present study was to construct a parentage verification system for iranian caspian horse. a total number of 45 caspian horse samples including 14 foals for parentage verification, 17 stallion and 14 mare for individual identification were genotyped. genomic dna was extracted from whole blood and the genotype were analysed by pcr procedure and using 7 microsatellite markers (aht04, hms03, hm...
secale montanum is one of the important perennial grasses growingnaturally in arid to semiarid pastures and rangelands, with a typical mediterraneanclimate, in northern and western iran at altitudes of 800-2900 m. in this paper, seedstorage protein profiles of nine wild populations of s. montanum from differentregions of iran and their phenotypically superior progenies as well as a multi-origin...
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