Cell wall proteins with sialidase activity are involved in carbohydrate assimilation, adhesion to mucosal surfaces, and biofilm formation. Gardnerella spp. inhabit the human vaginal microbiome encode up three enzymes, two of which suspected be cell associated. Here, we demonstrate that gene encoding extracellular NanH3 is found almost exclusively piotii closely related species genome sp. 3, its...

Extracellular vesicles (EVs) are lipid bilayer-enclosed nanosized particles released by all cell types during physiological as well pathophysiological processes to carry out diverse biological functions, including acting sources of cellular dumping, signalosomes and mineralisation nanoreactors. The ability EVs perform specific functions is due their biochemical machinery. Among the components E...

Microplasts are large extracellular vesicles originating from migratory, invasive, and metastatic cancer cells. Here, to gain insight into the role of microplasts in progression, we performed a proteomic transcriptomic characterization isolated MCF-7 breast cells treated with macrophage-conditioned medium. These were found be viable, highly metabolically active, indicating that derived these no...

Protein evolution is not simply a race toward improved function. Because organisms compete for limited resources, fitness is also affected by the relative economy of an organism's proteome. Indeed, many abundant proteins contain relatively high percentages of amino acids that are metabolically less taxing for the cell to make, thus reducing cellular cost. However, not all abundant proteins are ...