Accurate, low-level measurement of 226Ra in high volume water samples requires rapid pre-concentration and robust separation techniques prior to measurement in order to comply with discharge limits and drinking water regulations. This study characterises the behaviour of 226Ra and interfering elements on recently developed TK100 (Triskem International) extraction chromatography resin. Distribut...

In the conventional multiphase flow, immiscible having a liquid-liquid interface, two liquids are merged in flow path to form interface. The two-phase separation mixed solution has property of phase-separating from one phase phases by changing temperature/pressure, and phase-separates into upper lower batch-type vessel. By while sending this micro-space region, interface is formed obtained. Thi...

A rapid method based upon extraction chromatography was developed for the separation of U and Pu from solutions of spent nuclear fuel for thermal ionization mass spectrometry. The method involves retention of Pu(iv) and U(vi) on a UTEVA column, thereby accomplishing a separation of these actinides from fission products. A separation of Pu from U was then achieved by selective elution of Pu. Two...

Methods available for the assay of 25-hydroxycalciferol in human serum are evaluated and compared to one another. Ethanol was chosen for use in the initial extraction procedure and rat-kidney cytosol as the binding protein, although good alternative methods are also available. We used silicic acid for chromatography and found this an essential step. Reproducibility was increased when, after "bo...

Atmospheric pressure chemical ionization-tandem mass spectrometry (APCI-MS/MS) was applied for the first time to the direct analysis of octamethylcyclotetrasiloxane (D4) and decamethylcyclopentasiloxane (D5) in gaseous matrixes without extraction or prior chromatographic separation. Mass spectrometric characteristics of both compounds under APCI conditions and their fragmentation behavior in MS...

A high performance liquid chromatography method for determining the levels of 4-pregnene-3-ones in tissue containing fat, e.g. in thymus is reported. Following the extraction with chloroform/methanol the fat has been separated from the steroidal-fraction by using disposable extraction-columns. The steroidal-fraction has been analysed in two separation systems, on a RP18-column with a methanol/w...

In this work, using benzo(a)pyrene (BaP) as marker, the analytical merits of on-line flow-injection cloud-point extraction (FI CPE), including preconcentration factor, extraction efficiency, sample throughput, and analysis time were evaluated by use of peroxyoxalate chemiluminescence (CL) detection. Moreover, by detailed discussion of several preconcentration conditions for traditional and on-l...

I. An electrophoretic method for the rapid separation of the phosphate esters of inositol is presented. 2. It is used, together with ion-exchange chromatography, to establish the presence of inositol diand triphosphates in hydrolysates of trichloroacetic acid residues of rat brain, liver, heart, kidney, and lung. 3. With the aid of a *lP marker, inositol diand triphosphates were also found in h...

We report a means of determining amoxicillin in bovine plasma by liquid chromatography with UV detection at 235 nm. Purification and concentration of extracts were accomplished by a tandem solid-phase extraction procedure with two reversed-phase columns. Separation of amoxicillin from interferences was improved by the incorporation of a crown ether in the solvent systems used both for the solid...

A large number of studies have suggested that polysaccharides, such as fucoidan and laminarin, in various seaweeds have significant biological properties. A different distribution of molecular weights is a prominent sign of many polysaccharides. Therefore, a simple, fast and reliable high-performance size-exclusion chromatography (HPSEC) method was proposed to separate fucoidan and laminarin fr...