Mesenchymal stromal cells (MSC) have shown their benefits in graft-versus-host disease (GVHD), with five unsettled matters: 1) MSCs expansion in medium with Fetal Bovine Serum (FBS) and its risk of xenoreaction; 2) The number of cells indicated for therapy that is relatively high, with the need to optimize the expansion, number and time wise; 3) The utilization of third party donors; 4) Culture...

Profilin has been implicated in cell motility and in a variety of cellular processes, such as membrane extension, endocytosis, and formation of focal complexes. In vivo, profilin replenish the pool of ATP-actin monomers by increasing the rate of nucleotide exchange of ADP-actin for ATP-actin, promoting the incorporation of new actin monomers at the barbed end of actin filaments. For this report...

In an experiment, 200 one-day-old broiler chickens (Ross 308) were used to investigate the effects of sumac fruit (Rhus coriaria L.) powder (SFP) on performance, plasma concentrations of total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-c), low density lipoprotein (LDL-c), very low density lipoprotein (VLDL-c) and plasma fasting blood sugar (FBS), as well as proportional ...

Endometrial stromal cell culture Endometrial biopsies were obtained by curettage from consenting women aged 18-40 in accordance with local ethics committee requirements. Endometrial stromal cells were isolated and cultured as described previously (1). All experiments were performed within the first three cell passages. To induce decidualization in vitro, hESCs were treated with 0.5 mM 8-Bromo-c...

PURPOSE To compare cryopreserved human corneal endothelial cells (HCECs) grown in human serum-supplemented media (HS-SM) with cryopreserved HCECs grown in fetal bovine serum-supplemented media (FBS-SM). METHODS Three pairs of human corneas from donors aged 8, 28, and 31 years were obtained from the eye bank. From each pair, one cornea was used to start a HCEC culture using HS-SM; the other co...

BACKGROUND Lead is a toxic element which causes acute, subacute or chronic poisoning through environmental and occupational exposure. The aim of this study was to investigate clinical and laboratory abnormalities of chronic lead poisoning among workers of a car battery industry. METHODS Questionnaires and forms were designed and used to record demographic data, past medical histories and clin...

Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia. Fetuses are probably exposed to pain and/or discomfort, so the current practice of fetal blood harvesting is inhumane. Apart from moral concerns, sever...

In this work we study discrete analogues of an exact sequence vector bundles introduced by M. Atiyah in 1957, associated to any smooth principal $G$-bundle $\pi:Q\rightarrow Q/G$. the original setting, splittings correspond connections on bundle $\pi$. The that consider here can be studied two different categories: category fiber with a (chosen) section, FBS, and local Lie groupoids, lLgpdC. FB...

BACKGROUND Cardiotocography (CTG) is worldwide the method for fetal surveillance during labour. However, CTG alone shows many false positive test results and without fetal blood sampling (FBS), it results in an increase in operative deliveries without improvement of fetal outcome. FBS requires additional expertise, is invasive and has often to be repeated during labour. Two clinical trials have...

Dear Editor, Soft tissue foreign bodies (FBs) are a common occurrence in emergency departments. Not all FBs are discovered during the initial patient encounter; several signs reveal the presence of a retained FB in a wound.1 Some FBs cause significant problems, including infl ammation, chronic pain and repeat visits, whereas some FBs are asymptomatic and remain undetected for months or years. W...