background: various approaches have been used in the attempts to improve the quality of frozen–thawed mouse sperms. according to literatures, it seems that hyaluronic acid (ha) has an important role on the permeability and motility of sperms and their interaction with gametes. objective: for evaluation of ha supplementation on sperm characteristics and fertilization capability, we investigated ...

Abstract Study question The impact of Conventional IVF versus ICSI on embryo morphokinetic is still scarce. Would proper selection fertilization method affect embryonic development in different subgroups? Summary answer In unexplained or tubal factor subgruops, convential was preferable resulting higher rate and better quality expressed by less discarded embryos. What known already requires a s...

The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

the host plant quality not only influences on herbivorous insects but also indirectly could impact on their natural enemies’ performance. the current study was conducted to determine the effect of different nitrogen fertilization levels of canola (brassica napus l.) on life table parameters of the predatory gall midge, aphidoletes aphidimyza rondani; diptera: cecidomyiidae, in feeding of mustar...

BACKGROUND Failed fertilization after IVF occurs in 10-20% of cycles. Conflicting results of rescue fertilization by ICSI have been reported. We therefore compared the success rate in terms of fertilization and pregnancy of cycles in which rescue ICSI was performed with those from a matched control group of primarily ICSI cycles. METHODS Unfertilized oocytes from IVF cycles with total fertili...

BACKGROUND In vitro fertilization (IVF) of eggs by frozen and thawed C57BL/6J mouse sperm is inhibited by dead sperm and enhanced by preincubation of the sperm in calcium-free medium. In other species, the presence of sperm killed by freezing and thawing has been associated with the generation of hydrogen peroxide. METHODOLOGY/PRINCIPAL FINDINGS The proportion of eggs fertilized by cryopreser...

With the introduction of intracytoplasmic sperm injection (ICSI), couples with severe male factor infertility have achieved fertilization and clinical pregnancy rates comparable to other in-vitro fertilization (IVF) patients. However, failure of fertilization still occurs in some patients despite the utilization of microsurgical sperm injection techniques. How such fertilization failure after I...

The physiological processes leading to enhanced growth of loblolly pine (Pinus taeda L.) following fertilization are not clearly understood. Part of the debate revolves around the temporal response of net photosynthetic rate (A(n)) to fertilization and whether the A(n) response is always positive. We measured light-saturated photosynthetic rate (A(sat)), dark respiration rate, growth and crown ...

BACKGROUND The aim of this retrospective study is to investigate the relevance of dividing oocytes and using some for traditional in vitro fertilization (IVF) and others for intracytoplasmic sperm injection (ICSI) as of the first IVF cycle in patients with unexplained infertility who have undergone 4 intrauterine insemination (IUI) cycles which produced no pregnancies. METHODS This retrospect...

This study was designed to investigate the influence of seasonal variations on the number of retrieved ova, fertilization rate, embryo quality rates and pregnancy rate in IVF cycles. Prospective cohort study was conducted on a total number of 2140 cycles in infertile patients undergoing their first IVF cycle between 2000 and 2007 in IVF policlinic. Chi2-test, Pearson's correlation coefficient a...