background: rapidly increasing possibilities of exposure to environmental extremely low-frequency electromagnetic fields (elf-emf) have become a topic of worldwide investigation. epidemiological and laboratory studies suggest that exposure to elf-emf may increase cancer risk therefore assessment of chromosomal damage in various cell lines might be of predictive value for future risk estimation....

introduction: the aim of this study was to compare the cytotoxicity of proroot mta (pmta), root mta (rmta) and portland cement (pc) on human gingival fibroblasts (hgfs). materials and methods: the extracts of the test materials were placed close to human gingival fibroblasts (hgfs) within 96-well plates. cellular viability was assessed using mtt assay in different intervals (freshly mixed, 4, 2...

The angiogenic capacity of fibroblasts is maximized through the deliver plasmids and release proteins. Consequentially, endothelial cells proliferate organize, demonstrating potential system where vascularization required.

Epidermal growth factor (EGF) plays a critical role in fibroblasts by stimulating the production of collagen and supports cell renewal through the interaction between keratinocytes and fibroblasts. It is well known that the contractile activity of fibroblasts is required for the remodeling of the extracellular matrix (ECM), which contributes to skin elasticity. However, the role of EGF in the c...

Background: The aim of this study was to evaluate the role AT1 and AT2 receptors in a periodontal inflammation experimental model. Methods: Periodontal induced by LPS/Porphyromonas gingivalis. Maxillae, femur, vertebra were scanned using Micro-CT. Maxillae analyzed histopathologically, immunohistochemically, RT-PCR. Results: showed decreased BMD H compared with WT (p < 0.05). femur increased...

The purpose of this study was to examine regulation of collagen synthesis in human colon fibroblasts and compare the results from colon fibroblasts with those obtained in fibroblasts from human skin. The effects of interleukin-1 beta, tumour necrosis factor alpha, interferon gamma, transforming growth factor-beta, dexamethasone, and the calcium ionophore A23187 were investigated. All compounds ...

Hypertrophic scars are a common complication of burn injury, yet there no medications to prevent their formation. Myofibroblasts, key cell involved in scar formation, resistant apoptosis. We have previously shown that hydroxypyridone anti-fungals can inhibit myofibroblast transformation scar-derived fibroblasts. This study aimed investigate if these drugs target established myofibroblasts. Prim...

The aim of this study was to analyze the effect heme synthesis inhibition on cytoglobin expression and its correlation with keloid fibroblast viability proliferation. conducted primary culture fibroblasts. Heme in fibroblasts inhibited using succinyl acetone. We measured ALAD enzyme activity a colorimetric method; mRNA qRT-PCR; protein ELISA immunocytochemistry; MTT test; proliferation BrdU tes...

Hidradenitis suppurativa (HS) is a chronic, inflammatory skin disease characterized by massive immune cell infiltrate, tissue destruction and extensive fibrosis. We aimed to elucidate the role of fibroblasts in pathophysiology HS. Single-cell RNA-sequencing was performed on chronic lesional samples 8 HS patients controls. Ex vivo experiments were primary dermal from lesions (n=3). Fibroblast su...

Amelotin (AMTN) is a secretory calcium-binding phosphoprotein controlling the adhesion of epithelial cells to tooth surface, forming protective seal against oral cavity. It can be proposed that signals released upon dentinolysis increase AMTN expression in periodontal cells, thereby helping preserve seal. Support for this assumption comes from our RNA sequencing approach showing gingival fibrob...