The DNA-binding protein Fis frequently uses pairs of sites 7 or 11 base pairs (bp) apart. Two overlapping Fis sites separated by 11 bp are found in the Escherichia coli origin of chromosomal replication. Only one of these sites is bound by Fis at a time, so the structure is a molecular ̄ip± ̄op that could direct alternative ®ring of replication complexes in opposite directions. Alternatively, th...

The Hough transform approach accumulates evidence from the whole image in support a particular feature or shape, which in this implementation is restricted to circles. In contrast the FIS and ELS algorithms are based on local search techniques and are able to search for more complex shapes. The principal difference between the FIS and ELS method is that the ELS is based on a strongly edge based...

In this research, a fuzzy inference system (FIS) coupled with image processing technique was developed as a decision-support system for qualitative grading of milled rice. Two quality indices, namely degree of milling (DOM) and percentage of broken kernels (PBK) were first graded by rice processing experts into five classes. Then, images of the same samples were captured using a machine vision ...

OBJECTIVES Our primary aim was to assess how patients' characteristics, bleeding pattern, sonographic endometrial thickness (ET) and additional features at unenhanced ultrasound examination (UTVS) and at fluid instillation sonography (FIS) contribute to the diagnosis of intracavitary uterine pathology in women presenting with abnormal uterine bleeding (AUB). We further aimed to report the preva...

Modeling the ful llment of global properties like survivability is a challenging problem in unbounded systems such as Grids, peer-to-peer systems, or swarms. This paper proposes Folded Interaction Systems (FIS), an extension of the classic I-Systems framework, to overcome the modeling issues. FIS is applied to a case of survivability assessment in Grids and demonstrates the identi cation of ess...

FIS belongs to the group of small abundant DNA-binding proteins of Escherichia coli. We recently demonstrated that, in vivo, FIS regulates the expression of several genes needed for catabolism of sugars and nucleic acids, a majority of which are also transcriptionally regulated by cAMP-cAMP-receptor protein (CRP) complex. Here we provide evidence that FIS represses transcription of the crp gene...

BACKGROUND Passively acquired respiratory syncytial virus (RSV) neutralizing antibody protects against RSV-associated lower respiratory infections, but placental malaria (PM) and maternal hypergammaglobulinemia might interfere with transplacental immunoglobulin transport. METHODS We measured RSV plaque-reduction neutralization (PRN) antibody in 300 full-term maternal/cord serum pairs in 2 coh...

Bacteria and bacteriophages have evolved DNA modification as a strategy to protect their genomes. Mom protein of bacteriophage Mu modifies the phage DNA, rendering it refractile to numerous restriction enzymes and in turn enabling the phage to successfully invade a variety of hosts. A strong fortification, a combined activity of the phage and host factors, prevents untimely expression of mom an...

CspA, the most characterized member of the csp gene family of Escherichia coli, is highly expressed not only in response to cold stress, but also during the early phase of growth at 37°C. Here, we investigate at molecular level the antagonistic role played by the nucleoid proteins FIS and H-NS in the regulation of cspA expression under non-stress conditions. By means of both probing experiments...

Dickeya dadantii is a pathogen infecting a wide range of plant species. Soft rot, the visible symptom, is mainly due to the production of pectate lyases (Pels) that can destroy the plant cell walls. Previously we found that the pel gene expression is modulated by H-NS and FIS, two nucleoid-associated proteins (NAPs) modulating the DNA topology. Here, we show that relaxation of the DNA in growin...