To explain the experimentally observed effect of silver nanoparticles on fluorescence organic dyes and nonradiative intermolecular transfer electronic excitation energy in multilayer nanostructures, previously proposed theoretical model plasmon resonance spherical metals was used. The rates radiative (FRET) processes film structures with Ag were calculated for fluorescein rhodamine B molecules,...

We developed an experimental model to study the permeability of individual retinal vessels in vitro using microperfusion techniques adapted from kidney tubule studies. The retinal vessels were isolated by freehand dissection and mounted on a microperfusion apparatus. When inulin was perfused luminally, it was diluted to 80.2 +/- 2.3% of its initial concentration. However, no radioactive leak in...

Addition of sodium fluorescein to primary cultures of rat hepatocytes resulted in a rapid uptake of the dye by the hepatocytes and a subsequent accumulation in bile canaliculi-like structures. A similar distribution was obtained with fluorescein diacetate. Concentrations of Na-fluorescein accumulating within canaliculi varied over a wide range, often far exceeding that used in the medium. Ouaba...