Fluorescent materials are widely used in biological and material applications as probes for imaging or sensing; however, their customization is usually complicated without the support of an organic chemistry laboratory. Here, we present a straightforward method for the customization of BODIPY cores, which are among the most commonly used fluorescent probes. The method is based on the formation ...

fluorescent monitoring of dna amplification is the basis of real-time pcr. absolute quantification can be achieved using a standard curve method. the standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.the objective of the current study is to propose a mathematical model to assess the acceptability of pcr resulys.four commerci...

1. A direct fluorescent antibody (F.A.) tech­nique for diagnosis of Gonococci is de­scribed. 2. The results of applying F.A. method in the 270 female cases are presented and compared with the conventional method find­ings. 3. 220 cases were diagnosed by one or more methods at the first examinations. F .A. tests alone were positive in 44 ,eases and .F .A. direct diagnosis was made in a tot...

introduction: unlimited self renewal and potential capacity of embryonic stem cells (escs) in differentiating into a wide variety of cell types has made the cells an attractive source of donor cells for developmental studies and cell therapy. the aim of the present study was the evaluation of the transfection efficiency of pires2-egfp and pcdna3-hbdnf-v5 plasmids in cce es cells by the electrop...

The localization of Australia antigen in the liver by the fluorescent antibody technique (direct method) has been studied. Specific fluorescence was observed in the cytoplasm of liver cells. Two distribution patterns of fluorescent-positive cells, namely, a diffuse fluorescence in the liver lobules and a spotty distribution of fluorescent-positive cells, were observed.