Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes and separated nanoparticle-loaded liposomes from unencapsulated nanoparticles by size exclusion chromatography. Fluorescence correlation spectroscopy w...

recent investigations have been shown that inorganic nanomaterials including gold nanoparticles, nonporous and mesoporous silica nanoparticles, magneticnanoparticles, and quantum dots have shown great potential in bioimaging, targeted drug delivery, and cancer therapies. biocompatibility, ease of synthesis, and ease of surface functionalization are among the significant properties of nonporous ...

Excitation energy transfer from semi-conducting polymers to fluorescent dyes (or dye-labeled analytes) is commonly used in sensor applications, e.g., for DNA and protein sensing. Semi-conducting polymers show rather high absorption cross-sections and a high intra-chain mobility of the delocalized excitations leading to a high sensitivity for the excitation light. However, sensing of biological ...

Organic fluorescence agent designed into nanoparticle can significantly promote its fluorescent efficiency. In this pape, a novel fluorescein isothiocyanate labeling aminated agarose (FITC-AA) is prepared and tested as an effective fluorescent labeling agent. IR analysis and EA proved that FITC was grafted to the aminated agarose (AA) successfully. FITC-AA spontaneously formed nanoparticle with...

Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-...

Near-infrared fluorescence-enhanced diffuse optical tomography (fDOT) is used to localize tumors in mice using fluorescent nanoparticles as a blood pool contrast agent. The infrared dye DiR is loaded in the lipid core of nontargeted nanoparticles (DiR-lipidots) and injected systemically via the tail vein in mice bearing U87 tumors. Distribution and time-course of DiR-lipidots are followed using...