A highly sensitive assay procedure for 5,5-diphenylhydantoin (DPH) involves oxidation with alkaline permanganate to form benzophenone. This is then extracted with heptane, allowed to react with concentrated sulfuric acid, and the fluorescence of the reaction product is measured. The procedure appears to be fairly specific for DPH; 5.(p.hyd roxyphenyl)-5.phenylhydantoin (a DPH metabolite), pheno...

The calmodulin antagonist trifluoperazine and its analog chlorpromazine, both amphipaths, induced chemical germination of spores of various species, as do many surfactants. Cation load can greatly influence this response. Calmodulin antagonism does not seem to be involved. A new fluorometric assay for dipicolinic acid based on the fluorescence of the dipicolinic acid chelate of Tb3+ was found t...

A fluorometric substrate, 4-methylumbelliferyl-alpha-ketoside of N-acetylneuramide, was used directly on clinical specimens and infected tissue culture 24 h after inoculation for the detection of influenza viral neuraminidase. Viral neuraminidase was detected in infected tissue culture but not in clinical specimens. The sensitivity of the assay on tissue culture was 92%, and the specificity was...

We describe a kinetic, fluorometric assay for urinary Nacetyl-j3-glucosaminidase (EC 3.2.1.30) by measurement of the release of 4-methylumbelliferone (7-hydroxy-4-methylcoumarin). The method is simple, involving only a single reagent, and is applicable to the assay of other enzymes that hydrolyze similar fluorescent-labeled substrates. The enzyme distribution in human tissues and fluids is desc...

background: modulation of the immune response is an important strategy by which establish­ment and growth of hydatid cyst in the internal organs of human is warranted. induction of apop­tosis in the lymphocytes might be a considerable component. this study was designed to evaluate apoptotic impact of hydatid fluid (hf) on human lymphocytes. methods: human lymphocytes were treated with hydatid f...

Bullous pemphigoid (BP) has been associated with an increase in venous thromboembolism due to impaired fibrinolysis. As plasma levels of plasminogen activator inhibitor type 1 (PAI-1) are elevated BP patients, this represents one possible mechanism. We sought better understand the role autoantibodies inducing a prothrombotic state. first reviewed our previous RNA-seq database BP-IgG treated ker...

Three fluorometric leukocyte beta -glucosidase assays were compared for their ability to diagnose Gaucher's disease and identify carriers of the disorder: the acid beta-glucosidase assay of Beutler and Kuhl [2], a pH 5.5-sodium taurocholate-dependent assay and a new procedure which employs conduritol B epoxide, an active-site specific inhibitor of glucocerebrosidase. All three assays unambiguou...