Covalent binding of photoactivated aflatoxins to DNA in vitro under close-to-physiological conditions resulted in the formation of apurinic sites and in strand scission. Linearized pBR322 DNA was randomly fragmented, and supercoiled DNA was relaxed during the binding reaction. A primary amine facilitated fragmentation and relaxation. Quantitative measurements of relaxation revealed that the pro...

Jurkat leukemia cells induced to undergo apoptosis by treatment with an antibody against the Fas receptor have two annexin V (AV)-binding subpopulations: (a) single-positive cells that bind AV but not propidium iodide (PI); and (b) double-positive cells that bind AV and PI. The single-positive population is thought to represent an early stage of apoptosis. We have examined the relationship betw...

Covalent binding of photoactivated aflatoxins to DNA in vitro under close-to-physiological conditions resulted in the formation of apurinic sites and in strand scission. Linearized pBR322 DNA was randomly fragmented, and supercoiled DNA was relaxed during the binding reac tion. A primary amine facilitated fragmentation and relaxation. Quanti tative measurements of relaxation revealed that the p...

The length distributions of polymer fragments subjected to an elongational-flow-induced scission are profoundly affected by the fluid flow and the polymer bond strengths. In this paper, laminar elongational flow was used to induce chain scission of a series of circumference-programmed DNA nanotubes. The DNA nanotubes served as a model system for semiflexible polymers with tunable bond strength ...

Energy dissipation during wave propagation in fragmented geomaterials can be caused by independent movement of fragments leading to energy loss on their impact. By considering a pair of impacting fragments at times much greater than the period of their oscillations we show that at large time scale, the dynamics of the pair can be described by a linear viscous model with damping coefficient expr...

Usage of ureteral DJ stent is very common in urology practise. There are some complications according to the increased usage of ureteral DJ stent in the literature. Stent fragmentation is a rare complication of ureteral DJ stent. In this study we present a 53-year-old male patient who had previous stone surgery and a ureteral DJ stent applied to our polyclinic with complaint of 3-months-long ri...

DNA extracted from herbarium specimens is highly fragmented; therefore, it is crucial to use extraction protocols that retrieve short DNA molecules. Improvements in extraction and DNA library preparation protocols for animal remains have allowed efficient retrieval of molecules shorter than 50 bp. Here, we applied these improvements to DNA extraction protocols for herbarium specimens and evalua...

Activated DNA was immobilized in aldehyde-containing polyacrylamide gel for use in manufacturing the MAGIChip (microarrays of gel-immobilized compounds on a chip). First, abasic sites were generated in DNA by partial acidic depurination. Amino groups were then introduced into the abasic sites by reaction with ethylenediamine and reduction of the aldimine bonds formed. It was found that DNA coul...

introduction: the aim of this investigation was to study the developmental potential of human fragmented embryos to blastocyst stage and to evaluate thedeveloped blastocysts in the term of size, cellularity, the type of inner cell mass(icm), trophoetoderm (te) and the number of dead cells. material and methods: four to eight cell fragmented human embryos wereobtained from the embryology lab of ...

to be sure, all the industries try to be involved in globalization with a constant trend to find out ways to increase productivity across different functions within an organization to maintain competitive advantage world. pharmaceutical industries are not exceptional and further are based on fragmentation. so these kind of companies need to cope with several barriers such as silo mentality that...