Semen samples were collected from eight males of Goldfish (Carassius auratus gibelio) and cryopreserved using four extenders (Goldfish sperm extenders; GFSE 1– GFSE 4) supplemented with Methanol (MeOH) at concentration of 5%, 10% and 20%. Semen was diluted with ratio of 1:1 with extenders and frozen in liquid nitrogen vapor. Frozen sperms after 2 and 5 days were excluded from freezing. Experime...

BACKGROUND Sperm cryopreservation is the most effective method to preserve male fertility but this is normally used for motile spermatozoa. Thus, only motile spermatozoa are used for cryopreservation in most reproductive medicine centers worldwide. The immotile spermatozoa from some problematic patients are usually discarded, resulting in a missed opportunity of sterility cryopreservation for f...

Background Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. Ma...

Ram seminal plasma increases the fertility of frozen-thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP TrilEq (Triladyl with 0.5 mt of Equex STM paste added to each 100 mt) and heat-treated skim milk on longevity and percentages of progressively mot...

Abstract. The basal thermal state (frozen or thawed) of the Greenland Ice Sheet is under-constrained due to few direct measurements, yet knowledge this becoming increasingly important interpret modern changes in ice flow. first synthesis relied on inferences from widespread airborne and satellite observations numerical models, for which most underlying datasets have since been updated. Further,...

OBJECTIVE It is well known that fresh blastocyst transfer results in better pregnancy outcomes with a smaller number of transferred embryos compared with cleavage stage embryo transfer. However, in terms of frozen-thawed blastocyst transfer, only a few studies are available. We aimed to evaluate clinical outcomes of frozen-thawed embryo transfer (FET) with blastocysts. METHODS Retrospective a...

Lactic acid concentrations increased in refrigerated and freeze-thawed anaerobically stored ground beef. Bacterial counts were higher in refrigerated samples, but the ratios of gram-positive bacteria in refrigerated and freeze-thawed samples were the same. No differences in appearance or odor between refrigerated and freeze-thawed samples were noted after 2 days of aerobic storage. Initial lact...

A fertility trial was conducted comparing two methods of defining the optimum time to inseminate frozen boar semen. One hundred sixty-four gilts were inseminated, one-half at a fixed insemination time of 32 to 34 h after calculated onset of estrus and the remainder were inseminated on the basis of changes in the vaginal mucosal electrical resistance. Inseminations were made when the instrument ...

This data article contains supporting information regarding the research article entitled "Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm"(Mosca et. al., 2016) [1]. The combined effect of the permeant cryoprotectants agent dimethylacetamide and the non-permeant cryoprotectants agent trehalose on the quality of frozen-thawed chicken sem...

Fillet portions of freshly landed Atlantic cod, Gadus morhua, were either directly stored in ice or frozen for a briefperiod, thawed, and then stored in ice to determine and compare subsequent shelf lives so a retailer handling both products could be cognizant of the comparative per­ ishability of both product treatments. The frozen-thawed portions were found to have as long or a slightly longe...