Employing fluorescent antibodies for the detection of homologous plasma proteins in tissue sections, the distribution of plasma albumin, gamma-globulin, beta-lipoprotein, beta(1)-metal-combining globulin, and fibrinogen has been studied in the tissues of infants and children. Plasma albumin, gamma-globulin, and beta(1)-metal-combining globulin were found in many cells and particularly cell nucl...

OBJECTIVE To estimate different proteins and immunoglobulins as humoral immune response in patients with intracranial tumours. METHODS Cellulose acetate membrane electrophoresis for estimation of different proteins and radialimmunodiffusion (RID) for measurement of serum and CSF immunoglobulins were used. RESULTS Thirty seven patients with different types of tumours showed increase in serum...

Agglutinating substances having characteristics of naturally occurring macroglobulin antibodies to human Bence Jones proteins have been identified in human sera. By means of hemagglutination and hemagglutination inhibition techniques, common determinants have been demonstrated on the light (L) polypeptide chains of pooled normal human gamma(2)-globulin and on some Bence Jones proteins of group ...

Increased catabolism of plasma proteins by leakage into the gastrointestinal tract and subsequent digestion by the intestinal enzymes are frequently the causes of hypoproteinemia (1-5). The presence of plasma proteins has been demonstrated both in saliva and in the gastric and intestinal juice of normal individuals; therefore, gastrointestinal leakage of plasma proteins may account for a substa...

Immune gamma globulin has been coupled to ferritin by the diisocyanate method of Singer. The final product ("ferroglobulin") had approximately 13 per cent of its gamma globulin coupled to ferritin, and roughly half of its ferritin coupled to gamma globulin. The uncoupled gamma globulin could be removed by ultracentrifugal sedimentation of the free ferritin and the ferritin-antibody conjugates. ...

Sera containing Rh antibodies were taken from Rh-negative women at intervals during pregnancy and postpartum. A selection of these was fractionated on DEAE cellulose and by density gradient ultracentrifugation. The distribution among the immunoglobulin fractions gamma(SS), gamma(1)A, and gamma(1)M of Rh antibodies in the sera of Rh-negative women during pregnancy was analysed by these fractiona...

19S anti-gamma-globulins were isolated in a high state of purity from the sera of two patients with rheumatoid arthritis. Following reduction with ethyl mercaptan and alkylation by iodoacetamide, fragments were produced which retained the capacity to combine with 7S gamma-globulin. The fragments from one of the 19S anti-gamma-globulins agglutinated red cells coated with incomplete anti-Rh antib...

In most rapid radioimmunoassay methods, absorbents are used that disturb the equilibrium of the ligand-antibody interaction; thus the separation process must be rigidly timed. We show here that polyethylene glycol (mol wt. 6000) abolishes this constant. We measured gamma-globulin precipitation by polyethylene glycol (150g/liter) in a thyroxine radioimmunoassay system involving use of a sheep an...

Methyldopa-treated gamma globulin can be demonstrated serologically on either the red cell surface or on latex beads by the indirect antiglobulin reaction. The development of a positive antiglobulin reaction was related to methyldopa concentration and the length and temperature of incubation of methyldopa with protein and could be partially inhibited by the addition of albumin to the incubation...