Reaction-diffusion systems where one of the reagents (outer electrolyte) penetrates into a gel by diffusion and forms a precipitate with the other reagent (inner electrolyte) homogenized in the gel, are able to produce various complex precipitation patterns. The previously studied NaOH + AgNO3 and recently discovered CuCl2 + K3[Fe(CN)6] processes, (where the first reagent is the outer electroly...

The first pathway-specific step in the biosynthesis of the core membrane diether lipids in archaebacteria is the alkylation of the primary hydroxyl group in (S).glyc. eryl phosphate by geranylgeranyl diphosphate. The reaction is catalyzed by (S)-3-0·geranylgeranylglyceryl phosphate «S)-GGGP) synthase. The cytosolic enzyme was purified to homogeneity from the moderately thermophilic archae bacte...

Hemolysins purified from non-O1 Vibrio cholerae (non-O1 hemolysin) and a Vibrio cholerae O1, biotype El Tor (El Tor hemolysin) were investigated for their homology. The hemolysins were isolated from the culture supernatant fluids by ammonium sulfate precipitation and gel filtration on Sephadex G-100 columns. The purified hemolysins gave single bands with an identical mobility on conventional po...

This study investigates structural similarities in polyfluorosilicone (PFSi) gels made by different cross-linking procedures, using small-angle neutron scattering (SANS). The scattering properties of gels prepared from long and short precursor chains and of the un-cross-linked polymer solutions are reported. The gel samples were cross-linked by two methods, hydrosilylation, and condensation cur...

Interferon production was induced in mouse Ehrlich ascites tumor cells by infection with Newcastle disease virus. The interferon produced was purified by precipitation with ammonium sulfate, chromatography on carboxymethyl-Sephadex, treatment with blue dextran and polyethylene glycol, gel filtration on Bio-Gel P-60 and Bio-Gel P-200, chromatography on phosphocellulose, isoelectric focusing, and...

The cartilage matrix protein is a major non-collagenous protein in bovine cartilage. It was purified from a 5 M-guanidinium chloride extract of bovine tracheal cartilage by sequential CsCl-density-gradient centrifugation, gel chromatography in guanidinium chloride and differential precipitation. The molecular weight of the intact protein is 148 000, determined by sedimentation-equilibrium centr...

After being envenomated by the timber rattlesnake, a patient was found to have a platelet count of 5000 per microliter, prothrombin time and activated partial thromboplastin time both greater than 150 sec, plasma fibrinogen 0 mg/dl, and fibrinogen split products 2560 microgram/ml. However, this patient did not appear to have acute disseminated intravascular coagulation since coagulation factors...

For the preparation of proteins for proteome analysis, precipitation is frequently used to concentrate proteins and to remove interfering compounds. Various methods for protein precipitation are applied, which rely on different chemical principles. This study compares the changes in the protein composition of human blood platelet extracts after precipitation with ethanol (EtOH) or trichloroacet...

Abstract Different alumina samples prepared with sol–gel, chemical precipitation and hydrothermal synthesis were used as supports of Fe-promoted Rh-based catalysts for ethanol via CO hydrogenation. The characterized by means N 2 -adsorpotion, XRD, H -TPR, XPS, STEM, -TPD, DRIFTS, chemisorption. results indicated that the Al O 3 exhibited nano-fiber morphology constituted mixed crystal phases, w...

BACKGROUND We present the case of an 80-year-old woman who was admitted to hospital with an intermittent volvulus of the right colon. A total colectomy was performed. Initially, serum amylase and lipase increased concordantly, but after a few weeks amylase normalized (85 U/L), whereas lipase increased to 3764 U/L. This discrepancy and persistence of hyperlipasemia suggested a macromolecular for...